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An investigation of protein conformation of cytochrome c by using cytochrome c insolubilized on to agarose gel.通过使用固定在琼脂糖凝胶上的细胞色素c来研究细胞色素c的蛋白质构象。
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本文引用的文献

1
Some changes in the reactivity of enzymes resulting from their chemical attachment to water-insoluble derivatives of cellulose.由于酶与不溶于水的纤维素衍生物的化学结合而导致的酶活性的一些变化。
Biochem J. 1968 May;107(5):669-74. doi: 10.1042/bj1070669.
2
HORSE HEART CYTOCHROME C. SPECTROPHOTOMETRIC TITRATION AND VISCOSITY CHANGES IN ALKALINE SOLUTION.马心细胞色素C。碱性溶液中的分光光度滴定及粘度变化
Biochemistry. 1964 Nov;3:1648-50. doi: 10.1021/bi00899a008.
3
THE 695-MMM. BAND OF FERRICYTOCHROME C AND ITS RELATIONSHIP TO PROTEIN CONFORMATION.细胞色素C的695毫微米谱带及其与蛋白质构象的关系。
Biochemistry. 1964 Aug;3:1045-9. doi: 10.1021/bi00896a006.
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Spectrum of horse-heart cytochrome c.马心细胞色素c的光谱
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The preparation and properties of ficin chemically attached to carboxymethylcellulose.化学连接到羧甲基纤维素上的无花果蛋白酶的制备及其性质
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Evidence for the existence of two functionally distinct forms cytochrome c manomer at alkaline pH.在碱性pH条件下存在两种功能不同形式的细胞色素c单体的证据。
J Biol Chem. 1965 Sep;240(9):3660-3.
7
The reversible unfolding of horse heart ferricytochrome c.马心脏铁细胞色素c的可逆去折叠
Biochemistry. 1968 Aug;7(8):2893-8. doi: 10.1021/bi00848a028.
8
The chemical attachment of chymotrypsin to water-insoluble polymers using 2-amino-4,6-dichloro-s-triazine.
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Water-insoluble derivatives of enzymes, antigens, and antibodies.酶、抗原和抗体的水不溶性衍生物。
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10
Changes in conformation of insolubilized trypsin and chymotrypsin, followed by fluorescence.
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一种研究温度对不溶性细胞色素c的695纳米波段影响的方法。

A method for investigating the effect of temperature on the 695 nm band of insoluble cytochrome c.

作者信息

Moore T A, Greenwood C

出版信息

Biochem J. 1975 Jul;149(1):169-77. doi: 10.1042/bj1490169.

DOI:10.1042/bj1490169
PMID:172068
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1165603/
Abstract

A method is described for computer analysis of simple spectrophotometric changes in particulate systems, and this has been applied to the bleaching of the 695 nm band of insoluble ferricytochrome c by temperature. The results show that insolubilization has no effect on the standard enthalpy change but lowers the value for the standard entropy change. This effect appears to be independent of the concentration of the gel matrix to which the cytochrome c is bound, but dependent on the ionic strength of the surrounding solution.

摘要

本文描述了一种用于计算机分析颗粒系统中简单分光光度变化的方法,并将其应用于通过温度对不溶性高铁细胞色素c的695nm波段进行漂白。结果表明,不溶性对标准焓变没有影响,但降低了标准熵变的值。这种效应似乎与细胞色素c所结合的凝胶基质浓度无关,但取决于周围溶液的离子强度。