一种研究温度对不溶性细胞色素c的695纳米波段影响的方法。
A method for investigating the effect of temperature on the 695 nm band of insoluble cytochrome c.
作者信息
Moore T A, Greenwood C
出版信息
Biochem J. 1975 Jul;149(1):169-77. doi: 10.1042/bj1490169.
Abstract
A method is described for computer analysis of simple spectrophotometric changes in particulate systems, and this has been applied to the bleaching of the 695 nm band of insoluble ferricytochrome c by temperature. The results show that insolubilization has no effect on the standard enthalpy change but lowers the value for the standard entropy change. This effect appears to be independent of the concentration of the gel matrix to which the cytochrome c is bound, but dependent on the ionic strength of the surrounding solution.
摘要
本文描述了一种用于计算机分析颗粒系统中简单分光光度变化的方法，并将其应用于通过温度对不溶性高铁细胞色素c的695nm波段进行漂白。结果表明，不溶性对标准焓变没有影响，但降低了标准熵变的值。这种效应似乎与细胞色素c所结合的凝胶基质浓度无关，但取决于周围溶液的离子强度。
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