Funovics M
Angiographie und Interventionelle Radiologie, Klinik für Radiodiagnostik, Medizinische Universität Wien.
Radiologe. 2007 Jan;47(1):53-61. doi: 10.1007/s00117-006-1452-x.
The molecular imaging of specific targets using optical methods is currently possible in vivo, in part due to the advances in imaging modalities (epifluorescence, fluorescent endoscopy, fluorescence mediated tomography, intravital fluorescence microscopy), and in part due to the development of better contrast media. These are composed of a suitable fluorochrome, usually with emission in the near infrared due to tissue penetration, as well as by molecular specific contrast media from ligands. The latest generation of contrast media is not fluorescent in its unactivated state. Fluorescence first occurs after contact with certain (e.g. disease specific) enzymes by which a minimally unspecific fluorescent background as well as molecular specificity is made possible.
目前，利用光学方法对特定靶点进行分子成像在体内是可行的，部分原因是成像方式（落射荧光、荧光内镜检查、荧光介导断层扫描、活体荧光显微镜检查）的进步，部分原因是更好的造影剂的开发。这些造影剂由合适的荧光染料组成，由于组织穿透性，通常在近红外区域发射荧光，还包括来自配体的分子特异性造影剂。最新一代造影剂在未激活状态下不发荧光。与某些（如疾病特异性）酶接触后才会产生荧光，由此实现了最低限度的非特异性荧光背景以及分子特异性。
