登革病毒诱导的辅助细胞因子有两条带有不同决定簇的多肽链。
Dengue virus-induced helper cytokine has two polypeptide chains which bear different determinants.
作者信息
Chaturvedi P, Mukherjee R, Chaturvedi U C, Mathur A
机构信息
Postgraduate Department of Microbiology, K.G. Medical College, Lucknow, India.
出版信息
Int J Exp Pathol. 1991 Dec;72(6):665-72.
Dengue type 2 virus (DV) induces generation of a T cell helper cytokine (HF) in mouse spleen which enhances the antigen-specific antibody plaque forming cell count in syngeneic mice. The present study was undertaken to investigate the molecular structure of HF. It was observed that the activity of HF was abrogated by treatment with reducing agents such as glutathione, ouabain or dithiothreitol (DTT) which cleave disulphide bonds, separating the polypeptide chains. The two polypeptide chains could be purified by high performance liquid chromatography of DTT treated HF. The individual chains had no helper activity, but it could be restored by mixing the two. One chain of the HF bonded to the DV-antigen coupled immunosorbent column and the other to the anti-I-Ak antibody coupled column. Thus, DV-induced HF is a disulphide bonded double chain structure, one chain having antigen and the other having I-A determinants; the presence of both chains is essential for helper activity.
登革2型病毒（DV）可诱导小鼠脾脏产生一种T细胞辅助细胞因子（HF），该因子可增强同基因小鼠中抗原特异性抗体斑块形成细胞的数量。本研究旨在探究HF的分子结构。研究发现，用能裂解二硫键、分离多肽链的还原剂（如谷胱甘肽、哇巴因或二硫苏糖醇（DTT））处理后，HF的活性被消除。通过对经DTT处理的HF进行高效液相色谱法，可纯化出两条多肽链。单独的链没有辅助活性，但将两者混合后活性可恢复。HF的一条链与DV抗原偶联免疫吸附柱结合，另一条与抗I-Ak抗体偶联柱结合。因此，DV诱导的HF是一种二硫键连接的双链结构，一条链具有抗原，另一条具有I-A决定簇；两条链的存在对辅助活性至关重要。
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