Mountzouris K C, Tsirtsikos P, Kalamara E, Nitsch S, Schatzmayr G, Fegeros K
Department of Animal Nutrition, Agricultural University of Athens, Iera Odos 75, 11855, Greece.
Poult Sci. 2007 Feb;86(2):309-17. doi: 10.1093/ps/86.2.309.
The aim of this work was to investigate the efficacy of a new multibacterial species probiotic in broiler nutrition. The probiotic contained 2 Lactobacillus strains, 1 Bifidobacterium strain, 1 Enterococcus strain, and 1 Pediococcus strain. Four hundred 1-d-old male Cobb broilers were allocated in 4 experimental treatments for 6 wk. The experimental treatments received a corn-soybean basal diet and were as follows: "control," with no other additions; "probiotic in feed and water," (PFW) with probiotic administered at 1 g/kg of feed for the whole period and in water on scheduled intervals during the first 4 wk; "probiotic in feed," (PF) with probiotic in feed as in PFW; and "antibiotic," (AB) with addition of avilamycin at 2.5 mg/kg of feed. Salinomycin Na was used as a coccidiostat. Each treatment had 5 replicates of 20 broilers. Treatment effects on parameters of broiler performance and cecal microbial ecology were determined. Broiler BW, feed intake, and feed conversion ratio were determined on a weekly and overall basis. Cecal microflora composition, concentration of volatile fatty acids, and activities of 5 bacterial glycolytic enzymes (alpha-galactosidase, beta-galactosidase, alpha-glucosidase, beta-glucosidase, and beta-glucuronidase) were determined at the end of the experiment. Overall, treatment PFW displayed a growth-promoting effect that did not differ from AB. Overall, feed conversion ratio in treatment AB was significantly better (P < or = 0.01) than the control treatment, whereas treatments PFW and PF were intermediate and not different from AB. Concentrations of bacteria belonging to Bifidobacterium spp., Lactobacillus spp., and gram-positive cocci were significantly (P < or = 0.05) higher in treatments PFW and PF compared with the control and AB treatments. Treatments PFW and PF had significantly higher specific activities of alpha-galactosidase and beta-galactosidase compared with the control and AB treatments. In conclusion, probiotic treatment PFW displayed a growth-promoting effect that was comparable to avilamycin treatment. In addition, treatments PFW and PF modulated the composition and, to an extent, the activities of the cecal microflora, resulting in a significant probiotic effect.
本研究旨在探究一种新型多菌种益生菌在肉鸡营养中的功效。该益生菌包含2株乳酸杆菌、1株双歧杆菌、1株肠球菌和1株片球菌。400只1日龄雄性科宝肉鸡被分配到4种实验处理中，为期6周。实验处理组接受玉米 - 大豆基础日粮，具体如下：“对照组”，无其他添加物；“饲料和水中添加益生菌”（PFW），在整个实验期间，饲料中添加益生菌的剂量为1 g/kg，前4周按预定间隔时间在饮水中添加；“饲料中添加益生菌”（PF），饲料中益生菌添加量与PFW相同；“抗生素组”（AB），饲料中添加2.5 mg/kg的阿维拉霉素。盐霉素钠用作抗球虫药。每个处理组有5个重复，每个重复20只肉鸡。测定处理对肉鸡生产性能参数和盲肠微生物生态的影响。每周和整个实验期结束时测定肉鸡体重、采食量和饲料转化率。在实验结束时测定盲肠微生物群落组成、挥发性脂肪酸浓度以及5种细菌糖酵解酶（α - 半乳糖苷酶、β - 半乳糖苷酶、α - 葡萄糖苷酶、β - 葡萄糖苷酶和β - 葡萄糖醛酸酶）的活性。总体而言，PFW处理组显示出与AB组相似的促生长效果。总体而言，AB处理组的饲料转化率显著优于对照组（P≤0.01），而PFW和PF处理组处于中间水平，与AB组无差异。与对照组和AB处理组相比，PFW和PF处理组中双歧杆菌属、乳酸杆菌属和革兰氏阳性球菌的浓度显著更高（P≤0.05）。与对照组和AB处理组相比，PFW和PF处理组的α - 半乳糖苷酶和β - 半乳糖苷酶的比活性显著更高。总之，益生菌PFW处理组显示出与阿维拉霉素处理相当的促生长效果。此外，PFW和PF处理组调节了盲肠微生物群落的组成，并在一定程度上调节了其活性，从而产生了显著地益生菌效应。
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