Francavilla F, Barbonetti A, Necozione S, Santucci R, Cordeschi G, Macerola B, Francavilla S
Department of Internal Medicine, Andrology Unit, University of L'Aquila, L'Aquila, Italy.
Int J Androl. 2007 Jun;30(3):174-81. doi: 10.1111/j.1365-2605.2006.00727.x. Epub 2007 Jan 19.
As little information exists on the semen variability in infertile men, this study aimed at analysing the within-subject variability of semen from men with infertile marriages included in an intrauterine insemination (IUI) programme. Five ejaculates from each of 436 men (2180 specimens) were analysed. The within-subject coefficients of variation (CV(w)) were high for all parameters (semen volume, sperm concentration, forward motility and combined parameters), ranging from 0.73 for the total motile sperm count to 0.27 for the semen volume. Nevertheless, within-subject fluctuations were smaller than the between-subject variability, as indicated by high Intraclass Correlation Coefficient (ICC) values, which, however, significantly lowered when <or=3 samples were analysed against five samples. Neither seasonality nor the length of abstinence between 3 and 7 days significantly affected within-subject semen variability, whereas total motile sperm count was significantly and negatively affected at 2 days of abstinence. In 253 cycles of double IUI, performed in two consecutive days, semen volume and parameters including volume decreased in the second sample, but semen quality improved in most cases of oligo- and/or asthenozoospermia. Useful information for clinical purposes is provided.
由于关于不育男性精液变异性的信息很少，本研究旨在分析纳入宫内人工授精（IUI）计划的不育男性婚姻中精液的个体内变异性。对436名男性（2180份样本）每人的5次射精进行了分析。所有参数（精液量、精子浓度、前向运动能力和综合参数）的个体内变异系数（CV(w)）都很高，从总活动精子数的0.73到精液量的0.27不等。然而，如高组内相关系数（ICC）值所示，个体内波动小于个体间变异性，不过，当分析的样本数≤3个与5个样本相比时，ICC值会显著降低。季节性以及禁欲3至7天的时长均未显著影响个体内精液变异性，而禁欲2天时总活动精子数受到显著负面影响。在连续两天进行的253个双IUI周期中，第二个样本的精液量和包括体积在内的参数有所下降，但在大多数少精子症和/或弱精子症病例中精液质量有所改善。提供了对临床有帮助的信息。
