Johnsson Nils, Johnsson Kai
Center for Molecular Biology of Inflammation, Cellular Biochemistry, University of Muenster, Von-Esmarch-Strasse 56, 48149 Muenster, Germany.
ACS Chem Biol. 2007 Jan 23;2(1):31-8. doi: 10.1021/cb6003977.
The visualization of biologically relevant molecules and activities inside living cells continues to transform cell biology into a truly quantitative science. However, despite the spectacular achievements in some areas of cell biology, the majority of cellular processes still operate invisibly, not illuminated by even our brightest laser beams. Further progress therefore will depend not only on improvements in instrumentation but also increasingly on the development of new fluorophores and fluorescent sensors to target these activities. In the following, we review some of the recent approaches to generating such sensors, the methods to attach them to selected biomolecules, and their applications to various biological problems.
对活细胞内生物相关分子和活性的可视化，正持续将细胞生物学转变为一门真正的定量科学。然而，尽管在细胞生物学的某些领域取得了惊人成就，但大多数细胞过程仍在无形地运作，即便我们最亮的激光束也无法照亮它们。因此，进一步的进展不仅将取决于仪器设备的改进，还越来越依赖于开发新的荧光团和荧光传感器来针对这些活性。在下文中，我们将回顾一些用于生成此类传感器的最新方法、将它们连接到选定生物分子的方法，以及它们在各种生物学问题中的应用。
