Terai Takuya, Nagano Tetsuo
Graduate School of Pharmaceutical Sciences, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan.
Curr Opin Chem Biol. 2008 Oct;12(5):515-21. doi: 10.1016/j.cbpa.2008.08.007.
Fluorescent probes based on small organic molecules have become indispensable tools in modern biology because they provide dynamic information concerning the localization and quantity of the molecules of interest, without the need of genetic engineering of the sample. In this review, following a brief outline of the principle of fluorescence imaging, we recount some recent achievements in the field of small-molecular fluorescent probes. First, probes for metal cations, including those suitable for two-photon imaging, are introduced. Next, methodologies to visualize proteases are discussed, with special emphasis on activity-based probes for use in vivo. All these probes have been confirmed to be applicable to cellular or in vivo imaging.
基于小分子有机化合物的荧光探针已成为现代生物学中不可或缺的工具，因为它们能够提供有关目标分子定位和数量的动态信息，而无需对样品进行基因工程操作。在本综述中，在简要概述荧光成像原理之后，我们讲述了小分子荧光探针领域的一些最新成果。首先，介绍了用于金属阳离子的探针，包括适用于双光子成像的那些探针。接下来，讨论了可视化蛋白酶的方法，特别强调了用于体内的基于活性的探针。所有这些探针均已证实可用于细胞成像或体内成像。
