Faculty of Chemistry, Materials and Bioengineering, Kansai University, Suita, Osaka 564-8680, Japan.
National Institute of Technology and Evaluation, Kisarazu, Ciba 292-0818, Japan.
Int J Mol Sci. 2021 Jan 27;22(3):1240. doi: 10.3390/ijms22031240.
We designed three types of RGD-containing barnacle adhesive proteins using self-assembling peptides. In the present study, three types of RGD-containing peptides were synthesized by solid-phase peptide synthesis, and the secondary structures of these peptides were analyzed by CD and FT-IR spectroscopy. The mechanical properties of peptide hydrogels were characterized by a rheometer. We discuss the correlation between the peptide conformation, and cell attachment and cell spreading activity from the viewpoint of developing effective tissue engineering scaffolds. We created a peptide-coated cell culture substrate by coating peptides on a polystyrene plate. They significantly facilitated cell adhesion and spreading compared to a non-coated substrate. When the RGDS sequence was modified at N- or C-terminal of R-Y, it was found that the self-assembling ability was dependent on the strongly affects hydrogel formation and cell adhesion caused by its secondary structure.
我们使用自组装肽设计了三种含有 RGD 的藤壶黏附蛋白。在本研究中，通过固相肽合成合成了三种含有 RGD 的肽，并通过 CD 和 FT-IR 光谱分析了这些肽的二级结构。通过流变仪对肽水凝胶的力学性能进行了表征。我们从开发有效的组织工程支架的角度讨论了肽构象与细胞附着和细胞扩展活性之间的相关性。我们通过在聚苯乙烯板上涂覆肽来创建涂覆肽的细胞培养底物。与未涂覆的基底相比，它们显著促进了细胞的黏附和铺展。当 RGDS 序列在 R-Y 的 N-或 C-末端修饰时，发现自组装能力取决于其二级结构强烈影响水凝胶的形成和细胞黏附。
