• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

B 亚群禽肉瘤和白血病病毒囊膜糖蛋白的 hr1 和融合肽区影响 pH 值依赖性膜融合。

The hr1 and fusion peptide regions of the subgroup B avian sarcoma and leukosis virus envelope glycoprotein influence low pH-dependent membrane fusion.

机构信息

McArdle Laboratory for Cancer Research, Department of Oncology, University of Wisconsin-Madison, Madison, Wisconsin, United States of America.

出版信息

PLoS One. 2007 Jan 24;2(1):e171. doi: 10.1371/journal.pone.0000171.

DOI:10.1371/journal.pone.0000171
PMID:17245447
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1764858/
Abstract

The avian sarcoma and leukosis virus (ASLV) envelope glycoprotein (Env) is activated to trigger fusion by a two-step mechanism involving receptor-priming and low pH fusion activation. In order to identify regions of ASLV Env that can regulate this process, a genetic selection method was used to identify subgroup B (ASLV-B) virus-infected cells resistant to low pH-triggered fusion when incubated with cells expressing the cognate TVB receptor. The subgroup B viral Env (envB) genes were then isolated from these cells and characterized by DNA sequencing. This led to identification of two frequent EnvB alterations which allowed TVB receptor-binding but altered the pH-threshold of membrane fusion activation: a 13 amino acid deletion in the host range 1 (hr1) region of the surface (SU) EnvB subunit, and the A32V amino acid change within the fusion peptide of the transmembrane (TM) EnvB subunit. These data indicate that these two regions of EnvB can influence the pH threshold of fusion activation.

摘要

禽肉瘤和白血病病毒 (ASLV) 包膜糖蛋白 (Env) 通过涉及受体引发和低 pH 融合激活的两步机制被激活以触发融合。为了鉴定能够调节该过程的 ASLV Env 区域，使用遗传选择方法鉴定了当用表达同源 TVB 受体的细胞孵育时，对低 pH 触发的融合具有抗性的亚组 B (ASLV-B) 病毒感染的细胞。然后从这些细胞中分离出亚组 B 病毒 Env (envB) 基因，并通过 DNA 测序进行表征。这导致鉴定了两种允许 TVB 受体结合但改变膜融合激活的 pH 阈值的常见 EnvB 改变：表面 (SU) EnvB 亚基的宿主范围 1 (hr1) 区域中的 13 个氨基酸缺失，以及跨膜 (TM) EnvB 亚基融合肽内的 A32V 氨基酸变化。这些数据表明，EnvB 的这两个区域可以影响融合激活的 pH 阈值。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/de52/1764858/f7a91ea61136/pone.0000171.g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/de52/1764858/0defed542f08/pone.0000171.g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/de52/1764858/a5dab1d798e1/pone.0000171.g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/de52/1764858/f7a91ea61136/pone.0000171.g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/de52/1764858/0defed542f08/pone.0000171.g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/de52/1764858/a5dab1d798e1/pone.0000171.g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/de52/1764858/f7a91ea61136/pone.0000171.g003.jpg

相似文献

1
The hr1 and fusion peptide regions of the subgroup B avian sarcoma and leukosis virus envelope glycoprotein influence low pH-dependent membrane fusion.B 亚群禽肉瘤和白血病病毒囊膜糖蛋白的 hr1 和融合肽区影响 pH 值依赖性膜融合。
PLoS One. 2007 Jan 24;2(1):e171. doi: 10.1371/journal.pone.0000171.
2
Low pH is required for avian sarcoma and leukosis virus Env-induced hemifusion and fusion pore formation but not for pore growth.禽肉瘤和白血病病毒Env诱导半融合和融合孔形成需要低pH值，但孔生长则不需要。
J Virol. 2004 Apr;78(7):3753-62. doi: 10.1128/jvi.78.7.3753-3762.2004.
3
Heptad repeat 2-based peptides inhibit avian sarcoma and leukosis virus subgroup a infection and identify a fusion intermediate.基于七肽重复序列2的肽抑制禽肉瘤和白血病病毒A亚群感染并鉴定出一种融合中间体。
J Virol. 2004 Dec;78(24):13430-9. doi: 10.1128/JVI.78.24.13430-13439.2004.
4
Alpharetrovirus envelope-receptor interactions.禽白血病病毒属包膜-受体相互作用
Curr Top Microbiol Immunol. 2003;281:107-36. doi: 10.1007/978-3-642-19012-4_3.
5
Mutational analysis of the candidate internal fusion peptide of the avian leukosis and sarcoma virus subgroup A envelope glycoprotein.禽白血病和肉瘤病毒A亚群包膜糖蛋白候选内部融合肽的突变分析
J Virol. 1998 Apr;72(4):3259-67. doi: 10.1128/JVI.72.4.3259-3267.1998.
6
The avian retrovirus avian sarcoma/leukosis virus subtype A reaches the lipid mixing stage of fusion at neutral pH.禽逆转录病毒A亚群禽肉瘤/白血病病毒在中性pH值下达到融合的脂质混合阶段。
J Virol. 2003 Mar;77(5):3058-66. doi: 10.1128/jvi.77.5.3058-3066.2003.
7
The central proline of an internal viral fusion peptide serves two important roles.病毒内部融合肽中的中心脯氨酸发挥着两个重要作用。
J Virol. 2000 Feb;74(4):1686-93. doi: 10.1128/jvi.74.4.1686-1693.2000.
8
Low pH is required for avian sarcoma and leukosis virus Env-dependent viral penetration into the cytosol and not for viral uncoating.低pH值是禽肉瘤和白血病病毒Env依赖性病毒渗透到细胞质中所必需的，而不是病毒脱壳所必需的。
J Virol. 2004 Oct;78(19):10433-41. doi: 10.1128/JVI.78.19.10433-10441.2004.
9
A charged second-site mutation in the fusion peptide rescues replication of a mutant avian sarcoma and leukosis virus lacking critical cysteine residues flanking the internal fusion domain.融合肽中带电荷的第二位点突变挽救了一种突变禽肉瘤和白血病病毒的复制，该病毒在内部融合结构域两侧缺乏关键的半胱氨酸残基。
J Virol. 2009 Sep;83(17):8575-86. doi: 10.1128/JVI.00526-09. Epub 2009 Jun 10.
10
Receptor-induced conformational changes in the SU subunit of the avian sarcoma/leukosis virus A envelope protein: implications for fusion activation.禽肉瘤/白血病病毒A包膜蛋白SU亚基中受体诱导的构象变化：对融合激活的影响。
J Virol. 2005 Mar;79(6):3488-99. doi: 10.1128/JVI.79.6.3488-3499.2005.

引用本文的文献

1
Peptide-based delivery of therapeutics in cancer treatment.基于肽的治疗药物在癌症治疗中的递送
Mater Today Bio. 2022 Mar 30;14:100248. doi: 10.1016/j.mtbio.2022.100248. eCollection 2022 Mar.
2
Single Amino Acids G196 and R198 in hr1 of Subgroup K Avian Leukosis Virus Glycoprotein Are Critical for Tva Receptor Binding.K亚群禽白血病病毒糖蛋白hr1中的单个氨基酸G196和R198对Tva受体结合至关重要。
Front Microbiol. 2020 Dec 16;11:596586. doi: 10.3389/fmicb.2020.596586. eCollection 2020.
3
The Bipartite Sequence Motif in the N and C Termini of gp85 of Subgroup J Avian Leukosis Virus Plays a Crucial Role in Receptor Binding and Viral Entry.

本文引用的文献

1
Na+/H+ exchanger type 1 is a receptor for pathogenic subgroup J avian leukosis virus.1型钠/氢交换体是致病性J亚群禽白血病病毒的一种受体。
Proc Natl Acad Sci U S A. 2006 Apr 4;103(14):5531-6. doi: 10.1073/pnas.0509785103. Epub 2006 Mar 27.
2
Avian sarcoma and leukosis virus-receptor interactions: from classical genetics to novel insights into virus-cell membrane fusion.禽肉瘤和白血病病毒-受体相互作用：从经典遗传学到病毒-细胞膜融合的新见解
Virology. 2006 Jan 5;344(1):25-9. doi: 10.1016/j.virol.2005.09.021.
3
Isolation of cell lines that show novel, murine leukemia virus-specific blocks to early steps of retroviral replication.
J 亚群禽白血病病毒 gp85 的 N 端和 C 端的二分序列基序在受体结合和病毒进入中起关键作用。
J Virol. 2020 Oct 27;94(22). doi: 10.1128/JVI.01232-20.
4
Retroviral host range extension is coupled with Env-activating mutations resulting in receptor-independent entry.逆转录病毒宿主范围的扩展伴随着 Env 激活突变，导致受体非依赖性进入。
Proc Natl Acad Sci U S A. 2017 Jun 27;114(26):E5148-E5157. doi: 10.1073/pnas.1704750114. Epub 2017 Jun 12.
5
Avian sarcoma leukosis virus receptor-envelope system for simultaneous dissection of multiple neural circuits in mammalian brain.用于同时剖析哺乳动物大脑中多个神经回路的禽肉瘤白血病病毒受体-包膜系统。
Proc Natl Acad Sci U S A. 2015 Jun 2;112(22):E2947-56. doi: 10.1073/pnas.1423963112. Epub 2015 May 19.
6
Structural characterization of a fusion glycoprotein from a retrovirus that undergoes a hybrid 2-step entry mechanism.一种逆转录病毒融合糖蛋白的结构特征，该病毒采用混合的 2 步进入机制。
FASEB J. 2013 Dec;27(12):5059-71. doi: 10.1096/fj.13-232371. Epub 2013 Sep 13.
7
Structures and mechanisms of viral membrane fusion proteins: multiple variations on a common theme.病毒膜融合蛋白的结构与机制：同一主题的多种变体
Crit Rev Biochem Mol Biol. 2008 May-Jun;43(3):189-219. doi: 10.1080/10409230802058320.
分离出对逆转录病毒复制早期步骤表现出新型鼠白血病病毒特异性阻断作用的细胞系。
J Virol. 2005 Oct;79(20):12969-78. doi: 10.1128/JVI.79.20.12969-12978.2005.
4
The receptor for the subgroup C avian sarcoma and leukosis viruses, Tvc, is related to mammalian butyrophilins, members of the immunoglobulin superfamily.C亚群禽肉瘤和白血病病毒的受体Tvc与哺乳动物嗜乳脂蛋白相关，嗜乳脂蛋白是免疫球蛋白超家族的成员。
J Virol. 2005 Aug;79(16):10408-19. doi: 10.1128/JVI.79.16.10408-10419.2005.
5
Imaging individual retroviral fusion events: from hemifusion to pore formation and growth.对单个逆转录病毒融合事件进行成像：从半融合到孔形成及生长
Proc Natl Acad Sci U S A. 2005 Jun 14;102(24):8728-33. doi: 10.1073/pnas.0501864102. Epub 2005 Jun 3.
6
Receptor-induced conformational changes in the SU subunit of the avian sarcoma/leukosis virus A envelope protein: implications for fusion activation.禽肉瘤/白血病病毒A包膜蛋白SU亚基中受体诱导的构象变化：对融合激活的影响。
J Virol. 2005 Mar;79(6):3488-99. doi: 10.1128/JVI.79.6.3488-3499.2005.
7
The many mechanisms of viral membrane fusion proteins.病毒膜融合蛋白的多种机制。
Curr Top Microbiol Immunol. 2005;285:25-66. doi: 10.1007/3-540-26764-6_2.
8
Heptad repeat 2-based peptides inhibit avian sarcoma and leukosis virus subgroup a infection and identify a fusion intermediate.基于七肽重复序列2的肽抑制禽肉瘤和白血病病毒A亚群感染并鉴定出一种融合中间体。
J Virol. 2004 Dec;78(24):13430-9. doi: 10.1128/JVI.78.24.13430-13439.2004.
9
A study of low pH-induced refolding of Env of avian sarcoma and leukosis virus into a six-helix bundle.一项关于低pH诱导禽肉瘤和白血病病毒Env重折叠成六螺旋束的研究。
Biophys J. 2004 Nov;87(5):3291-8. doi: 10.1529/biophysj.104.047696. Epub 2004 Aug 31.
10
Sequential roles of receptor binding and low pH in forming prehairpin and hairpin conformations of a retroviral envelope glycoprotein.受体结合和低pH值在逆转录病毒包膜糖蛋白形成前发夹和发夹构象中的顺序作用。
J Virol. 2004 Aug;78(15):8201-9. doi: 10.1128/JVI.78.15.8201-8209.2004.