玉米adh1 基因附近 DNA 中的限制位点多态性极高。
Exceptionally High Levels of Restriction Site Polymorphism in DNA near the Maize Adh1 Gene.
机构信息
Department of Genetics, University of California, Berkeley, California 94720.
出版信息
Genetics. 1983 Nov;105(3):733-43. doi: 10.1093/genetics/105.3.733.
Restriction maps have been prepared for the chromosomal region near seven biochemically and genetically distinct maize alcohol dehydrogenase-1 (Adh1) alleles using a small cDNA probe for Adh1. Five restriction sites spanning about 4 kb in and near the Adh1 transcription unit appear identical in all seven alleles. Outside this conserved region, variation in restriction site position is the rule. Six of the seven alleles are distinguishable, and the alleles appear to fall into four groups. The DNA flanking the 1S-type alleles seems to share no restriction site homology with the DNA near the 1F-type alleles. Several hypotheses are put forward to explain how such high levels of polymorphism could have arisen in a species that has been domesticated for only about 10,000 years.
已使用 Adh1 的小 cDNA 探针为靠近七个生化和遗传上不同的玉米醇脱氢酶-1 (Adh1) 等位基因的染色体区域准备了限制图谱。跨越 Adh1 转录单位内和附近约 4 kb 的 5 个限制位点在所有七个等位基因中似乎相同。在这个保守区域之外，限制位点位置的变化是规则。七个等位基因中的六个是可区分的，并且这些等位基因似乎分为四组。1S 型等位基因侧翼的 DNA 似乎与 1F 型等位基因附近的 DNA 没有共享限制位点同源性。提出了几种假设来解释在一个仅被驯化了约 10000 年的物种中，如何产生如此高水平的多态性。
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