玉米乙醇脱氢酶-1基因中的DNA甲基化
DNA methylation in the Alcohol dehydrogenase-1 gene of maize.
作者信息
Walbot V, Warren C
机构信息
Department of Biological Sciences, Stanford University, CA 94305-5020.
出版信息
Plant Mol Biol. 1990 Jul;15(1):121-5. doi: 10.1007/BF00017729.
Using a battery of methylation-sensitive restriction enzymes, cytosine methylation at 23 sites in a 7.6 kb region surrounding the Alcohol dehydrogenase-1 (Adh1) gene was measured in DNA prepared from immature maize cobs. Both the 5' upstream region and the entire coding region were hypomethylated in the two alleles examined. Methylation in Adh1 is independent of changes in Mutator transposable element methylation. The role of DNA methylation in Adh1 gene regulation is discussed.
使用一组对甲基化敏感的限制性内切酶，在从未成熟玉米穗轴制备的DNA中，测量了围绕乙醇脱氢酶-1（Adh1）基因的7.6 kb区域内23个位点的胞嘧啶甲基化情况。在所检测的两个等位基因中，5'上游区域和整个编码区域均呈低甲基化状态。Adh1中的甲基化与Mutator转座元件甲基化的变化无关。文中讨论了DNA甲基化在Adh1基因调控中的作用。
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