Department of Genetics and Development, University of Illinois, Urbana, Illinois 61801.
Genetics. 1984 Jul;107(3):435-46. doi: 10.1093/genetics/107.3.435.
Linkage relationships of nine enzyme loci; aconitase (Acon ), esterase (Est), glucosephosphate isomerase A and B ( Gpi), glycerate-2-dehydrogenase (G2dh), malic enzyme (Me ), phosphoglycerate kinase (Pgk), phosphoglucomutase (Pgm ) and superoxide dismutase (Sod), were investigated in sunfishes (Lepomis, Centrarchidae). Reciprocal F(1) hybrids produced from crosses between green sunfish (Lepomis cyanellus) and redear sunfish ( L. microlophus) were backcrossed with each of the two parental species. A three-point linkage map comprising G2dh, Pgk and Sod is reported. The frequencies of recombination between G2dh and Pgk and between Pgk and Sod are estimated as 45.3 and 24.7%. The remaining six loci assort independently. Possible linkage conservation and homology of this linkage group with those of other vertebrate species are discussed.
研究了太阳鱼属(Lepomis,Centrarchidae)中九个酶位点的连锁关系:柠檬酸合酶(Acon)、酯酶(Est)、葡萄糖磷酸异构酶 A 和 B(Gpi)、甘油醛-2-脱氢酶(G2dh)、苹果酸酶(Me)、磷酸甘油激酶(Pgk)、磷酸葡萄糖变位酶(Pgm)和超氧化物歧化酶(Sod)。从绿太阳鱼(Lepomis cyanellus)和红耳太阳鱼(L. microlophus)杂交产生的正反交 F1 杂种与两个亲本种中的每一个进行回交。报道了一个包含 G2dh、Pgk 和 Sod 的三点连锁图谱。估计 G2dh 和 Pgk 之间以及 Pgk 和 Sod 之间的重组频率分别为 45.3%和 24.7%。其余六个位点独立分配。讨论了该连锁群与其他脊椎动物物种的连锁保守性和同源性。