葡萄糖对黑腹果蝇淀粉酶基因表达的抑制作用。
Glucose Repression of Amylase Gene Expression in DROSOPHILA MELANOGASTER.
机构信息
Department of Biology, University of Ottawa, Ottawa, Ontario, Canada K1N 6N5.
出版信息
Genetics. 1986 Sep;114(1):137-44. doi: 10.1093/genetics/114.1.137.
Abstract
We have previously shown that dietary glucose can reduce amylase activity in both adults and larvae of Drosophila; this reduction in enzyme activity reflects a reduction in the quantity of amylase protein, rather than an inhibition of enzyme activity. Here, we report that we have now defined conditions in which the repressive effect of glucose can be greater than 100-fold. Moreover, this repression is partially counteracted by the addition of exogenous cyclic AMP. We also show that there is a direct correlation between changes in amylase activity and changes in the amount of translatable mRNA as assayed in microinjected Xenopus oocytes. This means that the glucose repression is occurring at a pretranslational stage.
摘要
我们之前已经表明，膳食葡萄糖可以降低果蝇成虫和幼虫的淀粉酶活性；这种酶活性的降低反映了淀粉酶蛋白数量的减少，而不是酶活性的抑制。在这里，我们报告说，我们现在已经确定了葡萄糖的抑制作用可以超过 100 倍的条件。此外，这种抑制作用部分被添加的外源性环 AMP 抵消。我们还表明，在微注射非洲爪蟾卵母细胞中测定的淀粉酶活性变化与可翻译 mRNA 量的变化之间存在直接相关性。这意味着葡萄糖的抑制作用发生在翻译前阶段。
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