钴氯化物显性抗性在盘基网柄菌中的表征及其在准性遗传分析中的应用。
Characterization of Dominant Resistance to Cobalt Chloride in DICTYOSTELIUM DISCOIDEUM and Its Use in Parasexual Genetic Analysis.
机构信息
Genetics Department, Research School of Biological Sciences, The Australian National University, P.O. Box 475, Canberra City, A.C.T. 2601, Australia.
出版信息
Genetics. 1978 Sep;90(1):37-47. doi: 10.1093/genetics/90.1.37.
Abstract
Strains of Dictyostelium discoideum resistant to cobaltous chloride have been isolated at a frequency of approximately 10(-6). The resistant strains have one of three phenotypes, recessive to wild type, dominant to wild type and dominant to wild type but requiring the presence of cobaltous chloride to maintain resistance. Strains carrying a dominant cobaltous chloride resistance mutation and a recessive growth temperature-sensitive mutation can be mixed with wild-type haploid lines and then subjected to selection so that only diploid lines survive. Differential sensitivity to cycloheximide has also been observed. Hypersensitivity to cycloheximide in combination with dominant cobaltous chloride resistance provides a means of selecting diploids without the use of temperature-sensitive mutations.
摘要
已从对氯化钴具有抗性的盘基网柄菌（Dictyostelium discoideum）菌株中以约 10(-6)的频率分离出抗性菌株。这些抗性菌株具有三种表型之一，对野生型为隐性，对野生型为显性，但需要存在氯化钴才能维持抗性。携带显性钴抗性突变和隐性生长温度敏感突变的菌株可以与野生型单倍体系混合，然后进行选择，以便只有二倍体系存活。还观察到对环己亚胺的差异敏感性。环己亚胺与显性钴抗性的超敏性为选择二倍体而不使用温度敏感突变提供了一种手段。
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1
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J Bacteriol. 1969 Jul;99(1):65-9. doi: 10.1128/jb.99.1.65-69.1969.
2
Plaque-size mutants of the cellular slime mould Dictyostelium discoideum.
J Gen Microbiol. 1968 Sep;53(2):181-6. doi: 10.1099/00221287-53-2-181.
3
Evidence for mitotic recombination in the cellular slime mold Dictyostelium discoideum.
Proc Natl Acad Sci U S A. 1974 Oct;71(10):4025-6. doi: 10.1073/pnas.71.10.4025.
4
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