在 DICTYOSTELIUM DISCOIDEUM 的有性遗传分析中使用抗制霉菌素突变。
Use of Nystatin-Resistant Mutations in Parasexual Genetic Analysis in DICTYOSTELIUM DISCOIDEUM.
机构信息
Department of Microbiology, State University of New York, Stony Brook, New York 11794.
出版信息
Genetics. 1983 Jun;104(2):271-7. doi: 10.1093/genetics/104.2.271.
Abstract
Nystatin-resistant mutations exhibit extreme sensitivity to 1.3 mm coumarin. The mutations fall into three complementation groups so it is possible to select for nonallelic mutations conferring sensitivity to coumarin by selection on nystatin-containing nutrient agar plates. Complementation between such coumarin-sensitive mutations allows the selection of diploids on coumarin-containing nutrient agar. Two of the nystatin resistance genes, nysB and nysC, have been mapped tentatively to the previously unmarked linkage group V.
摘要
耐制霉菌素突变体对 1.3 毫米香豆素表现出极度的敏感性。这些突变体分为三个互补群，因此可以通过在含有制霉菌素的营养琼脂平板上进行选择来筛选赋予香豆素敏感性的非等位基因突变体。这些香豆素敏感突变体之间的互补作用允许在含有香豆素的营养琼脂上选择二倍体。两个制霉菌素抗性基因 nysB 和 nysC 已被暂定映射到以前未标记的连锁群 V 上。
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