Loomis W F
J Bacteriol. 1969 Jul;99(1):65-9. doi: 10.1128/jb.99.1.65-69.1969.
Three classes of temperature-sensitive mutants of the cellular slime mold Dictyostelium discoideum have been isolated. One class contains strains able to grow at 22 C but not at 27 C. Cells of these strains can develop into sorocarps at both temperatures. Another class contains strains which can grow at both temperatures but can only develop at the lower temperature. The third class contains strains unable to grow or develop at 27 C. Those strains whose development is temperature-sensitive appear to carry mutations which affect the cells only during the period of aggregation before the construction of a multicellular sorocarp. When pairs of growth-temperature-sensitive (GTS) strains develop in mixed aggregates, temperature-resistant (TR) cells are formed at a frequency of about 10(-4). These TR cells transmit the phenotype in a relatively stable hereditary fashion. However, temperature-sensitive segregants can be isolated from TR strains even after six clonal passages. Mixed incubation of pairs of morphologically aberrant GTS strains was found to give rise to TR progeny which develop normally. These progeny clones independently segregate morphologically aberrant strains and temperature-sensitive strains. The results indicate that several temperature-sensitive and morphological mutations are recessive and nonidentical.
已分离出细胞黏菌盘基网柄菌的三类温度敏感突变体。一类包含能够在22℃生长但不能在27℃生长的菌株。这些菌株的细胞在这两个温度下都能发育成子实体。另一类包含在两个温度下都能生长但只能在较低温度下发育的菌株。第三类包含在27℃下无法生长或发育的菌株。那些发育对温度敏感的菌株似乎携带了仅在构建多细胞子实体之前的聚集期影响细胞的突变。当成对的生长温度敏感（GTS）菌株在混合聚集体中发育时，温度抗性（TR）细胞以约10^(-4)的频率形成。这些TR细胞以相对稳定的遗传方式传递表型。然而，即使经过六次克隆传代，仍可从TR菌株中分离出温度敏感的分离株。发现形态异常的GTS菌株对混合培养会产生正常发育的TR后代。这些后代克隆独立分离出形态异常的菌株和温度敏感的菌株。结果表明，几种温度敏感和形态突变是隐性且不相同的。
