果蝇体细胞核纯合缺失的活力。
Viability of Homozygous Deficiencies in Somatic Cells of DROSOPHILA MELANOGASTER.
机构信息
Centro de Biología Molecular, CSIC, Universidad Autónoma de Madrid, Madrid-34, Spain.
出版信息
Genetics. 1979 Mar;91(3):443-53. doi: 10.1093/genetics/91.3.443.
Abstract
The viability of cells made homozygous for different deficiencies by induced mitotic recombination was examined. The deficiencies varied in length from two to 30 polytene chromosome bands and were distributed over the five major chromosome arms. Among a sample of 30, ten deficiencies were cell viable. Our results show that 12% of the genome is necessary for cell survival, supporting previous estimates of about 5,000 genes in the genome of Drosophila.
摘要
通过诱导有丝分裂重组使细胞成为纯合不同缺失型，检测了这些细胞的生存能力。缺失的长度从两条到 30 条多线染色体带不等，分布在五个主要染色体臂上。在 30 个样本中，有 10 个缺失型是细胞存活的。我们的结果表明，基因组的 12%是细胞生存所必需的，这支持了先前对果蝇基因组中约 5000 个基因的估计。
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