一种开发用于分析丹参、三七和复方丹参的统一高效液相色谱法。
A uniform HPLC method developed for the analysis of Salvia miltiorrhiza, Panax notoginseng, and Fufang Danshen.
作者信息
Zeng Guifeng, Liu Jianxun, Wang Longxing, Xu Qing, Xiao Hongbin, Liang Xinmiao
机构信息
Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China.
出版信息
J Chromatogr Sci. 2006 Nov-Dec;44(10):591-5. doi: 10.1093/chromsci/44.10.591.
Fufang Danshen (FFDS) is a famous typical Chinese complex prescription, which is mainly composed of Radix Salvia miltiorrhiza Bunge (SM) and Radix Panax notoginseng (PN). An HPLC method is developed to analyze SM, PN, and FFDS effectively; the effective analysis is achieved by using a gradient elution procedure with a mobile phase consisting of acetonitrile and 0.025% aqueous phosphoric acid (v/v). Through this method, 33 peaks in FFDS are clearly exhibited, and the components that make up the 33 peaks in FFDS are evaluated. Also, the chemical ingredients are compared between the single herbs (SM and PN) and the complex prescription (FFDS). The result indicate that the chemical ingredients in FFDS are not simply a combination of SM and PN. In addition, the HPLC method is suitable for the routine quality control of SM, PN, and FFDS, which could present a uniform quality control method for single medicines and one of the most commonly used Traditional Chinese Medicine-complex prescriptions.
复方丹参（FFDS）是一种著名的典型中药复方制剂，主要由丹参（SM）和三七（PN）组成。开发了一种高效液相色谱（HPLC）方法来有效分析丹参、三七和复方丹参；通过使用由乙腈和0.025%磷酸水溶液（v/v）组成的流动相进行梯度洗脱程序来实现有效分析。通过该方法，复方丹参中的33个峰清晰显示，并对构成复方丹参中这33个峰的成分进行了评估。此外，还比较了单味药（丹参和三七）与复方制剂（复方丹参）中的化学成分。结果表明，复方丹参中的化学成分并非简单地是丹参和三七的组合。此外，该HPLC方法适用于丹参、三七和复方丹参的常规质量控制，可为单味药和最常用的中药复方制剂之一提供统一的质量控制方法。
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