Liang Jie-Ping, Liu Zhong-Zheng, Peng Wei, Su Wei-Wei
School of Life Sciences, Sun Yat-sen University, Guangzhou 510275, China.
Zhong Yao Cai. 2012 Nov;35(11):1854-8.
To establish the HPLC fingerprint of Compound Xueshuantong Capsule.
Dionex Acclaim 120 C18 column (4.6 mm x 150 mm, 3 microm) was used with acetonitrile (A) and 0.05% phosphoric acid (B) in gradient elution mode. The elution profile was:0-50 min (15%-->34% A), 50-95 min (34%-->75% A); The detective wavelength was 203 nm and 270 nm. The column temperature was set at 25 degrees C and the flow rate was 1.0 mL/min.
In the established fingerprint,42 common peaks covering 4 medicinal materials were detected, and 23 chemical compounds were identified by RRLC/MS/MS and DAD spetra.
The method can be used for quality control of Compound Xueshuantong Capsule with great precision, accuracy and good reproducibility.
建立复方血栓通胶囊的高效液相色谱指纹图谱。
采用戴安公司的Acclaim 120 C18色谱柱（4.6 mm×150 mm，3 µm），以乙腈（A）和0.05%磷酸（B）为流动相，进行梯度洗脱。洗脱程序为：0～50分钟（15%→34%A），50～95分钟（34%→75%A）；检测波长为203 nm和270 nm。柱温设定为25℃，流速为1.0 mL/min。
在所建立的指纹图谱中，共检测到42个来源于4种药材的共有峰，通过快速液相色谱/质谱/质谱联用仪（RRLC/MS/MS）和二极管阵列检测器（DAD）光谱鉴定出23种化合物。
该方法可用于复方血栓通胶囊的质量控制，具有较高的精密度、准确性和良好的重复性。
