用于检测针对肺支原体的免疫球蛋白类别特异性抗体的固相微放射免疫测定法。
Solid-phase micro-radioimmunoassay to measure immunoglobulin class-specific antibody to Mycoplasma pulmonis.
作者信息
Taylor G
出版信息
Infect Immun. 1979 Jun;24(3):701-6. doi: 10.1128/iai.24.3.701-706.1979.
Abstract
A solid-phase radioimmunoassay (RIA) was used for the detection and quantitation of immunoglobulin class-specific antibodies to Mycoplasma pulmonis in mouse sera. The RIA was found to be more sensitive than the single radial haemolysis (SRH) test for detecting antibodies in sera from mice at intervals after inoculation with M. pulmonis, since antibody was detected in 26 out of 29 serum samples by RIA but in only 8 out of 29 samples by SRH. A method is described for quantitating immunoglobulin class-specific antibody in test sera by reference to a standard serum. The RIA should be useful for the study of the relative importance of various immunoglobulin classes in resistance to mycoplasma infections.
摘要
采用固相放射免疫测定法（RIA）检测和定量小鼠血清中针对肺支原体的免疫球蛋白类特异性抗体。结果发现，在接种肺支原体后的不同时间间隔检测小鼠血清中的抗体时，RIA比单向辐射溶血（SRH）试验更灵敏，因为通过RIA在29份血清样本中的26份检测到了抗体，而通过SRH仅在29份样本中的8份检测到抗体。本文描述了一种通过参考标准血清来定量检测血清中免疫球蛋白类特异性抗体的方法。RIA对于研究各种免疫球蛋白类在抵抗支原体感染中的相对重要性应该是有用的。
相似文献
1
Solid-phase micro-radioimmunoassay to measure immunoglobulin class-specific antibody to Mycoplasma pulmonis.用于检测针对肺支原体的免疫球蛋白类别特异性抗体的固相微放射免疫测定法。
Infect Immun. 1979 Jun;24(3):701-6. doi: 10.1128/iai.24.3.701-706.1979.
2
Detection of antibodies to Mycoplasma pulmonis by an enzyme-linked immunosorbent assay.通过酶联免疫吸附测定法检测肺支原体抗体。
Infect Immun. 1978 Oct;22(1):161-70. doi: 10.1128/iai.22.1.161-170.1978.
3
Synovial immunoglobulin and antibody in vaccinated and nonvaccinated calves challenged with Mycoplasma bovis.接种和未接种疫苗的犊牛在感染牛支原体后滑膜中的免疫球蛋白和抗体
Can J Comp Med. 1981 Jan;45(1):92-6.
4
Determination of IgG, IgM, and IgA antibodies to Mycoplasma pneumoniae by an indirect staphylococcal radioimmunoassy.用间接葡萄球菌放射免疫分析法测定肺炎支原体的IgG、IgM和IgA抗体。
Med Microbiol Immunol. 1978 May 26;165(1):29-41. doi: 10.1007/BF02121230.
5
A solid phase micro-radioimmunoassay to detect minute amounts of Ig class specific anti-viral antibody in a mouse model system.一种用于在小鼠模型系统中检测微量Ig类特异性抗病毒抗体的固相微放射免疫测定法。
J Immunol Methods. 1975 Oct;8(4):319-30. doi: 10.1016/0022-1759(75)90053-8.
6
Measurement of antibody to Ureaplasma urealyticum by an enzyme-linked immunosorbent assay and detection of antibody responses in patients with nongonococcal urethritis.通过酶联免疫吸附测定法检测解脲脲原体抗体及非淋菌性尿道炎患者抗体反应的检测
J Clin Microbiol. 1983 Feb;17(2):288-95. doi: 10.1128/jcm.17.2.288-295.1983.
7
8
Immunoglobulin class- and subclass-specific responses to Mycoplasma pulmonis in sera and secretions of naturally infected Sprague-Dawley female rats.自然感染的斯普拉格-道利雌性大鼠血清和分泌物中对肺支原体的免疫球蛋白类和亚类特异性反应。
Infect Immun. 1991 Jun;59(6):2181-5. doi: 10.1128/iai.59.6.2181-2185.1991.
9
Radioimmunoassays that use 125I-labeled protein A for determination of Histoplasma capsulatum-specific immunoglobulin G, A, and M class antibodies in histoplasmosis.使用125I标记的蛋白A进行放射免疫测定，以检测组织胞浆菌病中荚膜组织胞浆菌特异性免疫球蛋白G、A和M类抗体。
J Clin Microbiol. 1981 Jan;13(1):66-72. doi: 10.1128/jcm.13.1.66-72.1981.
10
A solid-phase radioimmunoassay for detection of antibodies against Mycoplasma pneumoniae.一种用于检测抗肺炎支原体抗体的固相放射免疫测定法。
J Clin Lab Immunol. 1983 Aug;11(4):209-13.
引用本文的文献
1
2
Class-specific antibody responses to Mycoplasma pulmonis in sera and lungs of infected and vaccinated mice.感染和接种疫苗小鼠的血清及肺脏中针对肺支原体的特异性类别抗体反应。
Infect Immun. 1980 Sep;29(3):1160-8. doi: 10.1128/iai.29.3.1160-1168.1980.
3
4
A comparison of three vaccines against respiratory syncytial virus in calves.三种抗犊牛呼吸道合胞病毒疫苗的比较。
J Hyg (Lond). 1984 Oct;93(2):251-61. doi: 10.1017/s0022172400064779.
5
Experimental pneumonia in gnotobiotic calves produced by respiratory syncytial virus.呼吸道合胞病毒引发的悉生小牛实验性肺炎
Br J Exp Pathol. 1984 Feb;65(1):19-28.
6
Infection of gnotobiotic calves with a bovine and human isolate of respiratory syncytial virus. Modification of the response by dexamethasone.用牛源和人源呼吸道合胞病毒分离株感染无菌小牛。地塞米松对反应的影响。
Arch Virol. 1984;79(1-2):67-77. doi: 10.1007/BF01314304.
7
Monoclonal antibodies protect against respiratory syncytial virus infection in mice.单克隆抗体可保护小鼠免受呼吸道合胞病毒感染。
Immunology. 1984 May;52(1):137-42.
8
Isotype commitment of B cells and dissemination of the primed state after mucosal stimulation with Mycoplasma pulmonis.肺炎支原体黏膜刺激后B细胞的同种型定向及致敏状态的扩散
Infect Immun. 1985 Aug;49(2):428-34. doi: 10.1128/iai.49.2.428-434.1985.
本文引用的文献
1
Protective Effect of Vaccines in Experimental Mycoplasma pneumoniae Disease.疫苗在实验性肺炎支原体病中的保护作用。
Infect Immun. 1970 Jun;1(6):559-65. doi: 10.1128/iai.1.6.559-565.1970.
2
The examination of nasal mucus for antibodies to mycoplasma mycoides.检测鼻黏液中针对丝状支原体的抗体。
Vet Rec. 1969 Apr 19;84(16):417-8. doi: 10.1136/vr.84.16.417.
3
A new mycoplasma species isolated from pneumonic lungs of calves (Mycoplasma dispar sp. nov.).从患肺炎的犊牛肺中分离出的一种新支原体物种（新种支原体 dispar）。
J Med Microbiol. 1970 Feb;3(1):111-23. doi: 10.1099/00222615-3-1-111.
4
Antibodies in bronchial secretions following natural infection with Mycoplasma pneumoniae.肺炎支原体自然感染后支气管分泌物中的抗体。
Acta Pathol Microbiol Scand B Microbiol Immunol. 1971;79(4):599-605. doi: 10.1111/j.1699-0463.1971.tb03818.x.
5
A radioimmunoprecipitation test for detection of Mycoplasma pneumoniae antibody.一种用于检测肺炎支原体抗体的放射免疫沉淀试验。
Proc Soc Exp Biol Med. 1973 May;143(1):97-105. doi: 10.3181/00379727-143-37261.
6
Comparison of direct and indirect solid-phase microradioimmunoassays for the detection of viral antigens and antiviral antibody.用于检测病毒抗原和抗病毒抗体的直接和间接固相微放射免疫测定法的比较。
Appl Microbiol. 1973 Apr;25(4):567-73. doi: 10.1128/am.25.4.567-573.1973.
7
Immune response of pathogen-free mice inoculated intranasally with Mycoplasma pulmonis.经鼻内接种肺炎支原体的无特定病原体小鼠的免疫反应。
J Immunol. 1974 Jan;112(1):124-36.
8
Microtiter solid-phase radioimmunoassay for hepatitis B antigen.乙型肝炎抗原的微量滴定板固相放射免疫测定法。
Appl Microbiol. 1973 Oct;26(4):478-84. doi: 10.1128/am.26.4.478-484.1973.
9
Studies on the adsorption of certain medium proteins to Mycoplasma gallisepticum and their influence on agglutination and haemagglutination reactions.某些培养基蛋白对鸡毒支原体的吸附及其对凝集和血凝反应影响的研究。
J Hyg (Lond). 1972 Jun;70(2):267-78. doi: 10.1017/s0022172400022324.
10
Effects of active and passive immunization on Mycoplasma pulmonis-induced pneumonia in mice.主动免疫和被动免疫对小鼠肺炎支原体诱导的肺炎的影响。
Immunology. 1976 May;30(5):611-8.
Zotero 插件