Sarkkinen H K, Meurman O H, Halonen P E
J Med Virol. 1979;3(4):281-9. doi: 10.1002/jmv.1890030406.
A solid-phase radioimmunoassay (RIA) has been developed for the detection of human rotavirus-specific IgA, IgG, and IgM antibodies. Nebraska calf diarrhea virus grown in LLC-MK2 cell cultures in the presence of trypsin was directly adsorbed onto polystyrene balls, and antibodies that attached to the virus-coated balls were detected by subsequent binding of 125I-labeled antibodies specific to human alpha, gamma or mu chains of human Iga, IgG, or IgM immunoglobulins. A total of 116 serum specimens from 58 adult patients were tested. Binding ratios between the positive and the negative serum varied between 5 and 15, occasionally being 20 or more in the IgA and IgG assays, but rarely exceeding 3 in the IgM assay. The RIA was found to be more sensitive in detecting antibodies to rotavirus than the complement fixation (CF) test, the RIA titers obtained being 50--100 times as high as the CF titers. The method described offers a possibility of evaluating the immune response to human rotavirus and of detecting recent infection.
已开发出一种固相放射免疫测定法（RIA）用于检测人轮状病毒特异性IgA、IgG和IgM抗体。在胰蛋白酶存在的情况下，在LLC-MK2细胞培养物中培养的内布拉斯加小牛腹泻病毒直接吸附到聚苯乙烯球上，通过随后结合针对人IgA、IgG或IgM免疫球蛋白的人α、γ或μ链的125I标记抗体来检测附着在病毒包被球上的抗体。对来自58名成年患者的总共116份血清标本进行了检测。阳性和阴性血清之间的结合率在5至15之间变化，在IgA和IgG测定中偶尔为20或更高，但在IgM测定中很少超过3。发现RIA在检测轮状病毒抗体方面比补体结合（CF）试验更敏感，获得的RIA效价是CF效价的50至100倍。所述方法提供了评估对人轮状病毒的免疫反应以及检测近期感染的可能性。
