Hemmati Shiva, Schmidt Thomas J, Fuss Elisabeth
Institut für Entwicklungs-und Molekularbiologie der Pflanzen, Heinrich-Heine-Universität Düsseldorf, Universitätsstrasse 1, Geb. 26.13.U1, D-40225 Düsseldorf, Germany.
FEBS Lett. 2007 Feb 20;581(4):603-10. doi: 10.1016/j.febslet.2007.01.018. Epub 2007 Jan 18.
A cDNA encoding a pinoresinol-lariciresinol reductase PLR (PLR-Lp1) was isolated from a cell culture of Linum perenne Himmelszelt accumulating the arylnaphthalene lignan justicidin B. The recombinant PLR-Lp1 prefers (+)-pinoresinol in the first reaction step, but (-)-lariciresinol in the second step. Therefore, it is the first PLR described with opposite enantiospecificity within the two reaction steps catalysed by PLRs. Hairy root lines transformed with an ihpRNAi construct to suppress plr gene expression show less mRNA accumulation for the plr-Lp1 gene and PLR enzyme activity. Justicidin B accumulation was reduced down to 24% in comparison to control lines showing the involvement of PLR-Lp1 in the biosynthesis of justicidin B.
从积累芳基萘木脂素异嗪皮啶B的多年生亚麻“天蓝色”细胞培养物中分离出一个编码松脂醇-落叶松脂醇还原酶PLR（PLR-Lp1）的cDNA。重组PLR-Lp1在第一个反应步骤中更倾向于（+）-松脂醇，但在第二个步骤中更倾向于（-）-落叶松脂醇。因此，它是在PLR催化的两个反应步骤中具有相反对映体特异性的首个被描述的PLR。用ihpRNAi构建体转化以抑制plr基因表达的毛状根系显示，plr-Lp1基因的mRNA积累减少，PLR酶活性降低。与对照系相比，异嗪皮啶B的积累降低至24%，表明PLR-Lp1参与异嗪皮啶B的生物合成。
