Fernandes Karl J L, Toma Jean G, Miller Freda D
Program in Developmental Biology, University of Toronto, Toronto, Ontario, Canada M5G 1X8.
Philos Trans R Soc Lond B Biol Sci. 2008 Jan 12;363(1489):185-98. doi: 10.1098/rstb.2006.2020.
We previously made the surprising finding that cultures of multipotent precursors can be grown from the dermis of neonatal and adult mammalian skin. These skin-derived precursors (SKPs) display multi-lineage differentiation potential, producing both neural and mesodermal progeny in vitro, and are an apparently novel precursor cell type that is distinct from other known precursors within the skin. In this review, we begin by placing these findings within the context of the rapidly evolving stem cell field. We then describe our recent efforts focused on understanding the developmental biology of SKPs, discussing the idea that SKPs are neural crest-related precursors that (i) migrate into the skin during embryogenesis, (ii) persist within a specific dermal niche, and (iii) play a key role in the normal physiology, and potentially pathology, of the skin. We conclude by highlighting some of the therapeutic implications and unresolved questions raised by these studies.
我们之前有一个惊人的发现，即多能前体细胞培养物可以从新生和成年哺乳动物皮肤的真皮中生长出来。这些皮肤衍生的前体细胞（SKP）具有多谱系分化潜能，在体外能产生神经和中胚层后代，并且显然是一种新型的前体细胞类型，与皮肤内其他已知的前体细胞不同。在这篇综述中，我们首先将这些发现置于快速发展的干细胞领域的背景下。然后我们描述了我们最近致力于理解SKP发育生物学的研究工作，讨论了SKP是与神经嵴相关的前体细胞这一观点，即（i）在胚胎发育过程中迁移到皮肤中，（ii）在特定的真皮生态位中持续存在，以及（iii）在皮肤的正常生理学乃至潜在病理学中发挥关键作用。最后，我们强调了这些研究引发的一些治疗意义和未解决的问题。
