Lévêque N, Amine I Lahlou, Cartet G, Hammani A B, Khazraji Y C, Lina B, Muyembe J J, Norder H, Chomel J J
Centre National de Référence des Entérovirus, Hôpital E. Herriot, Hospices Civils de Lyon, Lyon, France.
Eur J Clin Microbiol Infect Dis. 2007 Mar;26(3):199-202. doi: 10.1007/s10096-007-0265-9.
Reported here are two outbreaks of acute hemorrhagic conjunctivitis that occurred in the Democratic Republic of the Congo and in Morocco in the summers of 2003 and 2004, respectively, with a large impact on public health. Virus was isolated from the conjunctival swabs of 30 Congolese and 20 Moroccan patients. Enterovirus-specific cytopathic effect was observed in all samples. None of the strains could be typed using a conventional neutralization assay with the Melnick intersecting pools; however, by sequencing the VP1 region, the viruses could be identified as coxsackie A24 variants. Phylogenetic analysis of the 3C protease region revealed that these strains were closely related to each other as well as to genotype III isolates detected in Korea in 2002, thus proving their worldwide spread. This is the first report of an epidemic of acute hemorrhagic conjunctivitis due to a coxsackievirus A24 variant in Africa since 1987 and the first ever from Morocco.
本文报道了分别于2003年夏季和2004年夏季在刚果民主共和国和摩洛哥发生的两起急性出血性结膜炎疫情，对公共卫生造成了重大影响。从30名刚果患者和20名摩洛哥患者的结膜拭子中分离出病毒。在所有样本中均观察到肠道病毒特异性细胞病变效应。使用Melnick交叉池常规中和试验无法对任何菌株进行分型；然而，通过对VP1区域进行测序，这些病毒可被鉴定为柯萨奇A24变种。对3C蛋白酶区域的系统发育分析表明，这些菌株彼此密切相关，也与2002年在韩国检测到的III型基因型分离株密切相关，从而证明了它们在全球的传播。这是自1987年以来非洲首次关于由柯萨奇病毒A24变种引起的急性出血性结膜炎疫情的报告，也是摩洛哥有史以来的首次报告。
