García Prado E, García Gimenez M D, De la Puerta Vázquez R, Espartero Sánchez J L, Sáenz Rodríguez M T
Department of Pharmacology, Faculty of Pharmacy, University of Seville, c/ Profesor García González n. 2, 41012 Seville, Spain.
Phytomedicine. 2007 Apr;14(4):280-4. doi: 10.1016/j.phymed.2006.12.023. Epub 2007 Feb 12.
Uncaria tomentosa inner bark extract is a popular plant remedy used in folk medicine to treat tumor and inflammatory processes. In this study, the anti-tumoral effects of its pentacyclic alkaloid mitraphylline were investigated. Furthermore, its growth-inhibitory and cytotoxic effects on glioma GAMG and neuroblastoma SKN-BE(2) cell lines were studied using cyclophosphamide and vincristine as controls. A colter counter was used to determine viable cell numbers, followed by application of the tetrazolium compound [3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)2-(4-sulfophenyl)-2H-tetrazolium], inner salt, colorimetric method to evaluate cell viability in this cytotoxicity assay. Micromolar concentrations of mitraphylline (from 5 to 40 microM) inhibited the growth of both cell lines. It inhibited the growth of the two cell lines studied in a dose-dependent manner. The IC(50) values were 12.3 microM (30h) for SKN-BE(2) and 20 microM (48 h) for GAMG, respectively. This action suggests that mitraphylline is a new and promising agent in the treatment of human neuroblastoma and glioma.
毛钩藤内皮提取物是一种在民间医学中用于治疗肿瘤和炎症过程的常用植物药物。在本研究中，对其五环生物碱帽柱木碱的抗肿瘤作用进行了研究。此外，以环磷酰胺和长春新碱作为对照，研究了其对神经胶质瘤GAMG细胞系和神经母细胞瘤SKN - BE(2)细胞系的生长抑制和细胞毒性作用。使用血细胞计数器确定活细胞数量，随后应用四氮唑化合物[3-(4,5-二甲基噻唑-2-基)-5-(3-羧甲氧基苯基)-2-(4-磺基苯基)-2H-四氮唑]内盐比色法在该细胞毒性试验中评估细胞活力。微摩尔浓度的帽柱木碱（5至40 microM）抑制了两种细胞系的生长。它以剂量依赖的方式抑制了所研究的两种细胞系的生长。SKN - BE(2)的IC(50)值分别为12.3 microM（30小时）和GAMG的20 microM（48小时）。这一作用表明帽柱木碱是治疗人类神经母细胞瘤和神经胶质瘤的一种新的有前景的药物。
