Cattoir Vincent, Weill François-Xavier, Poirel Laurent, Fabre Laëtitia, Soussy Claude-James, Nordmann Patrice
Service de Bactériologie-Virologie, Hôpital de Bicêtre, Assistance Publique/Hôpitaux de Paris, Faculté de Médecine Paris-Sud, Université Paris XI, K.-Bicêtre, France.
J Antimicrob Chemother. 2007 Apr;59(4):751-4. doi: 10.1093/jac/dkl547. Epub 2007 Feb 16.
To detect the qnrA, qnrB and qnrS genes among Salmonella isolates received at the French National Reference Centre for Salmonella in Paris, France.
Antibiotic susceptibility was determined by disc diffusion for 499 Salmonella isolates including 320 Salmonella Typhimurium, 100 Salmonella Enteritidis and 79 Salmonella Hadar collected in 2002. Amplification with specific primers of qnrA, qnrB and qnrS genes was performed for all Salmonella Typhimurium, Salmonella Enteritidis and Salmonella Hadar isolates resistant to quinolones and for 17 additional isolates that produced expanded-spectrum beta-lactamases (ESBLs).
Prevalence of quinolone resistance was 3.75%, 11% and 79.7% for Salmonella Typhimurium, Salmonella Enteritidis and Salmonella Hadar serovars, respectively. A single isolate (0.2%) was qnrA-positive (QnrA1 determinant) being a Salmonella serovar Concord carrying also the ESBL gene bla(CTX-M-15). This strain was probably from East Africa. No qnrB or qnrS genes were identified.
Whereas plasmid-mediated quinolone resistance of the Qnr type is emerging in Enterobacteriaceae worldwide, it remains rare in Salmonella in France.
检测法国巴黎沙门氏菌国家参考中心收到的沙门氏菌分离株中的qnrA、qnrB和qnrS基因。
采用纸片扩散法对499株沙门氏菌进行药敏试验，这些菌株包括2002年收集的320株鼠伤寒沙门氏菌、100株肠炎沙门氏菌和79株哈达尔沙门氏菌。对所有耐喹诺酮类的鼠伤寒沙门氏菌、肠炎沙门氏菌和哈达尔沙门氏菌分离株以及另外17株产生超广谱β-内酰胺酶（ESBLs）的分离株，用qnrA、qnrB和qnrS基因的特异性引物进行扩增。
鼠伤寒沙门氏菌、肠炎沙门氏菌和哈达尔沙门氏菌血清型对喹诺酮类的耐药率分别为3.75%、11%和79.7%。一株分离株（0.2%）qnrA呈阳性（QnrA1决定簇），为携带ESBL基因bla(CTX-M-15)的康科德沙门氏菌血清型。该菌株可能来自东非。未鉴定出qnrB或qnrS基因。
虽然Qnr型质粒介导的喹诺酮类耐药性在全球范围内的肠杆菌科细菌中不断出现，但在法国的沙门氏菌中仍然罕见。
