Penna A, Fowler P, Bertoletti A, Guilhot S, Moss B, Margolskee R F, Cavalli A, Valli A, Fiaccadori F, Chisari F V
Cattedra Malattie Infettive, Università di Parma, Italy.
J Virol. 1992 Feb;66(2):1193-8. doi: 10.1128/JVI.66.2.1193-1198.1992.
In this study, we show that CD4+, hepatitis B virus (HBV) envelope-specific T-cell clones produced by stimulation with a particulate antigen preparation are able to recognize and kill not only autologous antigen-presenting cells incubated with exogenous HBV envelope antigens but also autologous HLA class II-positive cells expressing endogenously synthesized HBV envelope antigens following infection with recombinant vaccinia viruses or transfection with recombinant Epstein-Barr virus expression vectors. Experiments with lysosomotropic agents and brefeldin A suggest that the endosomal compartment is likely involved in the processing of endogenously synthesized viral proteins for recognition by CD4+ T cells. Our study indicates that HBV envelope-specific, HLA class II-restricted CD4+ cytotoxic T lymphocytes can potentially participate in the immune clearance of HBV-infected cells and the pathogenesis of hepatocellular injury in hepatitis B.
在本研究中，我们发现，用颗粒性抗原制剂刺激产生的CD4⁺、乙型肝炎病毒（HBV）包膜特异性T细胞克隆不仅能够识别并杀死与外源性HBV包膜抗原一起孵育的自体抗原呈递细胞，还能识别并杀死在用重组痘苗病毒感染或用重组爱泼斯坦-巴尔病毒表达载体转染后内源性合成HBV包膜抗原的自体HLA-II类阳性细胞。用溶酶体促渗剂和布雷菲德菌素A进行的实验表明，内体区室可能参与内源性合成的病毒蛋白的加工，以供CD4⁺ T细胞识别。我们的研究表明，HBV包膜特异性、HLA-II类限制性CD4⁺ 细胞毒性T淋巴细胞可能参与HBV感染细胞的免疫清除以及乙型肝炎中肝细胞损伤的发病机制。
