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单峰骆驼赛跑群体中的结核病暴发及受感染骆驼的快速血清学检测

Tuberculosis outbreak in a dromedary racing herd and rapid serological detection of infected camels.

作者信息

Wernery U, Kinne J, Jahans K L, Vordermeier H M, Esfandiari J, Greenwald R, Johnson B, Ul-Haq A, Lyashchenko K P

机构信息

Central Veterinary Research Laboratory, Dubai, UAE.

出版信息

Vet Microbiol. 2007 May 16;122(1-2):108-15. doi: 10.1016/j.vetmic.2007.01.012. Epub 2007 Jan 20.

Abstract

A recent outbreak of tuberculosis (TB) in a dromedary racing herd of 58 animals involved 3 infected animals. Disease was confirmed at necropsy by finding gross lesions from which Mycobacterium bovis (antelope type) was isolated. Sera collected from the camels in this herd were used to evaluate two new serological methods, Multiantigen Print Immunoassay (MAPIA) and rapid test (RT) developed using the lateral-flow technology, in comparison with the intradermal tuberculin tests. Antibodies were found in all three infected dromedaries by both RT and MAPIA, but not in the remaining 55 animals in the herd. With the limited number of animals tested in this study, the serological assays showed the potential for convenient, rapid, and accurate diagnosis of TB in live camels.

摘要

最近,在一个由58只动物组成的单峰骆驼赛跑群中爆发了结核病(TB),有3只动物被感染。通过尸检发现肉眼可见的病变,并从中分离出牛分枝杆菌(羚羊型),从而确诊了疾病。从该群体的骆驼身上采集血清,用于评估两种新的血清学方法,即多抗原印迹免疫测定法(MAPIA)和使用侧流技术开发的快速检测法(RT),并与皮内结核菌素试验进行比较。通过RT和MAPIA在所有三只感染的单峰骆驼中都发现了抗体,但在该群体中其余55只动物中未发现。由于本研究中测试的动物数量有限,血清学检测显示了在活骆驼中方便、快速且准确诊断结核病的潜力。

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