热休克反应在大肠杆菌K-12中mRNA稳定性中的作用
Role of the heat shock response in stability of mRNA in Escherichia coli K-12.
作者信息
Henry M D, Yancey S D, Kushner S R
机构信息
Department of Genetics, University of Georgia, Athens 30602.
出版信息
J Bacteriol. 1992 Feb;174(3):743-8. doi: 10.1128/jb.174.3.743-748.1992.
Abstract
The heat shock response in Escherichia coli involves extensive induction of the heat shock proteins, with the concomitant suppression of the synthesis of the non-heat shock proteins. While the induction of the heat shock proteins has been shown to occur primarily at the transcriptional level, the suppression of non-heat shock proteins is poorly understood. We have investigated the possibility that an increased decay of non-heat shock mRNAs is a means of decreasing the synthesis of non-heat shock proteins during the heat shock response. Heat shock response-defective strains were compared with wild-type controls by several criteria to evaluate both mRNA stability and the induction of enzymes known to be involved in mRNA turnover. Our results indicate that increased mRNA decay is not a mechanism used to regulate the synthesis of non-heat shock proteins.
摘要
大肠杆菌中的热休克反应涉及热休克蛋白的大量诱导，同时非热休克蛋白的合成受到抑制。虽然热休克蛋白的诱导主要发生在转录水平，但对非热休克蛋白的抑制了解甚少。我们研究了非热休克mRNA降解增加是否是热休克反应期间减少非热休克蛋白合成的一种方式。通过几个标准将热休克反应缺陷菌株与野生型对照进行比较，以评估mRNA稳定性和已知参与mRNA周转的酶的诱导情况。我们的结果表明，mRNA降解增加不是调节非热休克蛋白合成的机制。
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