Zappacosta Bruno, Manni Armando, Persichilli Silvia, Boari Antonia, Scribano Donata, Minucci Angelo, Raffaelli Luca, Giardina B, De Sole P
Centro di Ricerca e Formazione ad Alta Tecnologia nelle Scienze Biomediche Giovanni Paolo II, Università Cattolica del Sacro Cuore-Campobasso, Italy.
Clin Biochem. 2007 Jun;40(9-10):661-5. doi: 10.1016/j.clinbiochem.2007.01.011. Epub 2007 Jan 26.
Recent studies describe the potential use of biochemical markers in the evaluation of the severity of periodontitis; moreover, patients suffering from periodontitis frequently complain of halitosis (breath malodour), mainly depending on volatile compounds (e.g. hydrogen sulphide, methyl mercaptan, etc.) produced by anaerobic metabolism of oral bacteria and involving sulphur-containing amino acids. In this study, salivary sulphur compounds, such as cysteine, cysteinylglycine and glutathione and some markers of cellular damage (lactate dehydrogenase and aspartate amino transferase), were measured in periodontitis patients and correlated with the periodontal probing pocket's depth.
Twenty-two periodontitis patients and forty control subjects were studied for the salivary activities of lactate dehydrogenase and aspartate aminotransferase and cysteine, cysteinylglycine and glutathione concentrations. The periodontitis patients were divided into two subgroups based on the severity of periodontal disease, expressed as median periodontal probing pocket depth (> or <5 mm). Enzyme activities were measured by using an automated clinical analyzer; cysteine, cysteinylglycine and glutathione concentrations were measured by HPLC equipped with fluorescence detector.
A statistically significant increase of the salivary parameters level (cysteine, cysteinylglycine, glutathione, aspartate aminotransferase and lactate dehydrogenase) was found in the patient subgroup with periodontal probing pocket depth >5 mm, the salivary cysteine concentrations showing the most significant correlation.
Salivary cysteine, a direct precursor of hydrogen sulphide, could be considered reliable markers for the oral tissue damage severity in periodontitis patients.
近期研究描述了生化标志物在评估牙周炎严重程度方面的潜在用途；此外，牙周炎患者经常抱怨口臭，这主要取决于口腔细菌厌氧代谢产生的挥发性化合物（如硫化氢、甲硫醇等），且涉及含硫氨基酸。在本研究中，对牙周炎患者的唾液含硫化合物，如半胱氨酸、半胱氨酰甘氨酸和谷胱甘肽以及一些细胞损伤标志物（乳酸脱氢酶和天冬氨酸氨基转移酶）进行了测量，并将其与牙周探诊深度相关联。
对22名牙周炎患者和40名对照受试者的唾液乳酸脱氢酶和天冬氨酸氨基转移酶活性以及半胱氨酸、半胱氨酰甘氨酸和谷胱甘肽浓度进行了研究。根据牙周疾病的严重程度，以牙周探诊深度中位数（>或<5mm）为标准，将牙周炎患者分为两个亚组。酶活性通过自动临床分析仪进行测量；半胱氨酸、半胱氨酰甘氨酸和谷胱甘肽浓度通过配备荧光检测器的高效液相色谱法进行测量。
在牙周探诊深度>5mm的患者亚组中，唾液参数水平（半胱氨酸、半胱氨酰甘氨酸、谷胱甘肽、天冬氨酸氨基转移酶和乳酸脱氢酶）有统计学意义的升高，唾液半胱氨酸浓度显示出最显著的相关性。
唾液半胱氨酸作为硫化氢的直接前体，可被视为牙周炎患者口腔组织损伤严重程度的可靠标志物。
