Weissleder R, Lee A S, Khaw B A, Shen T, Brady T J
MGH-NMR Center, Department of Radiology, Massachusetts General Hospital, Charlestown.
Radiology. 1992 Feb;182(2):381-5. doi: 10.1148/radiology.182.2.1732953.
The synthesis and in vivo antigen targeting of a novel iron oxide compound were studied. A monocrystalline iron oxide nanoparticle (MION) was synthesized that contains a small (mean diameter, 2.9 nm +/- 0.9) single crystal core, passes through capillary membranes, and exhibits superparamagnetism. The MION was attached to antimyosin Fab (R11D10) and used for immunospecific magnetic resonance (MR) imaging of cardiac infarcts One hour after intravenous administration of MION-R11D10 in rats (100 mumol/kg), a marked decrease in the signal intensity of infarcted myocardium was observed. Immunohistochemical correlation confirmed the specific binding of the immunoconjugate to infarcted, but not to normal, myocardium. No decrease in cardiac signal intensity was observed when unconjugated MION was administered intravenously. The results indicate the feasibility of immunospecific MR imaging in living organisms.
研究了一种新型氧化铁化合物的合成及其体内抗原靶向性。合成了一种单晶氧化铁纳米颗粒（MION），其包含一个小的（平均直径，2.9 nm±0.9）单晶核心，可穿过毛细血管膜，并表现出超顺磁性。将MION连接到抗肌球蛋白Fab（R11D10）上，并用于心脏梗死的免疫特异性磁共振（MR）成像。在大鼠静脉注射MION-R11D10（100 μmol/kg）1小时后，观察到梗死心肌的信号强度明显降低。免疫组织化学相关性证实免疫缀合物与梗死心肌而非正常心肌特异性结合。静脉注射未缀合的MION时，未观察到心脏信号强度降低。结果表明免疫特异性MR成像在活生物体中的可行性。
