Prakash Om, Kumari Kirti, Lal Rup
Molecular Biology Laboratory, Department of Zoology, University of Delhi, Delhi 110 007, India.
Int J Syst Evol Microbiol. 2007 Mar;57(Pt 3):527-531. doi: 10.1099/ijs.0.64456-0.
A phenanthrene- and citronellol-degrading bacterium, strain RLD-1(T), was isolated from the fly ash dumping site of a thermal power plant in Delhi, India. The 16S rRNA gene sequence indicated that this strain belongs to the genus Pseudomonas; high levels of sequence similarity were found with respect to Pseudomonas citronellolis DSM 50332(T) (98.9 %), Pseudomonas jinjuensis DSM 16612(T) (97.6 %) and Pseudomonas nitroreducens DSM 14399(T) (97.5 %). Phylogenetic analysis based on 16S rRNA gene sequences placed the strain within the clade represented by these three strains. Strain RLD-1(T) showed low levels of DNA-DNA hybridization with respect to P. citronellolis DSM 50332(T) (36 %), P. jinjuensis DSM 16612(T) (4 %) and P. nitroreducens (13.7 %). Strain RLD-1(T) can also be distinguished from these three strains on the basis of several biochemical and physiological attributes. The novel strain contained high levels of cellular fatty acids 18 : 1omega7c, 16 : 0 and 16 : 1omega7c, along with 10 : 0 3-OH and 12 : 0 3-OH. Thus, strain RLD-1(T) represents a novel species of the genus Pseudomonas, for which the name Pseudomonas delhiensis sp. nov. is proposed. The type strain is RLD-1(T) (=MTCC 7601(T)=CCM 7361(T)).
从印度德里一家热电厂的粉煤灰倾倒场分离出一株能降解菲和香茅醇的细菌，菌株RLD-1(T)。16S rRNA基因序列表明该菌株属于假单胞菌属；与香茅醇假单胞菌DSM 50332(T)（98.9%）、晋州假单胞菌DSM 16612(T)（97.6%）和硝基还原假单胞菌DSM 14399(T)（97.5%）具有高度的序列相似性。基于16S rRNA基因序列的系统发育分析将该菌株置于由这三个菌株代表的进化枝内。菌株RLD-1(T)与香茅醇假单胞菌DSM 50332(T)（36%）、晋州假单胞菌DSM 16612(T)（4%）和硝基还原假单胞菌（13.7%）的DNA-DNA杂交水平较低。菌株RLD-1(T)也可根据几种生化和生理特性与这三个菌株区分开来。该新菌株含有高水平的细胞脂肪酸18 : 1ω7c、16 : 0和16 : 1ω7c，以及10 : 0 3-OH和12 : 0 3-OH。因此，菌株RLD-1(T)代表假单胞菌属的一个新物种，为此提出名为德里假单胞菌（Pseudomonas delhiensis）的新种。模式菌株为RLD-1(T)（=MTCC 7601(T)=CCM 7361(T)）。
