在位内膜与异位内膜（子宫内膜异位症）之间基因表达的c-DNA微阵列分析比较
Comparison of c-DNA microarray analysis of gene expression between eutopic endometrium and ectopic endometrium (endometriosis).
作者信息
Mettler L, Salmassi A, Schollmeyer T, Schmutzler A G, Püngel F, Jonat W
机构信息
Department of Obstetrics & Gynaecology, University Hospitals Schleswig-Holstein, Campus Kiel, Michaelisstr. 16, 24105, Kiel, Germany.
出版信息
J Assist Reprod Genet. 2007 Jun;24(6):249-58. doi: 10.1007/s10815-007-9116-y. Epub 2007 Mar 1.
Abstract
PROBLEM
As recent studies have suggested abnormalities in the regulation of specific genes in the development of endometriosis, we investigated differentially expressed genes in endometriosis compared to endometrium.
METHOD OF STUDY
Gene expression profiles using the Atlas microarray were performed in endometriotic tissue and endometrium. Nine of the 13 genes of endometriotic tissue showed an up-regulation in relation to endometrium and four of the 13 genes a down-regulation.
RESULTS
Of the 1176 genes on the Atlas Human 1,2 array, only 13 differentially expressed identical genes were detected after repeating the gene analysis three times.
CONCLUSION
According to our c-DNA analysis some differentially expressed genes may be involved in the pathogenesis of endometriosis. An imbalance in the genes responsible for the reproductive process may lead to a decrease in embryo implantation in patients with endometriosis.
摘要
问题
近期研究表明子宫内膜异位症发展过程中特定基因的调控存在异常，因此我们研究了与子宫内膜相比，子宫内膜异位症中差异表达的基因。
研究方法
使用Atlas微阵列对子宫内膜异位症组织和子宫内膜进行基因表达谱分析。子宫内膜异位症组织的13个基因中有9个相对于子宫内膜呈上调，13个基因中有4个呈下调。
结果
在Atlas Human 1,2阵列上的1176个基因中，经过三次重复基因分析后，仅检测到13个差异表达的相同基因。
结论
根据我们的c-DNA分析，一些差异表达的基因可能参与子宫内膜异位症的发病机制。负责生殖过程的基因失衡可能导致子宫内膜异位症患者胚胎着床减少。
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