Phillips M, Greenberg J
Department of Medicine, St. Vincent's Medical Center of Richmond, Staten Island, NY 10310.
Clin Chem. 1992 Jan;38(1):60-5.
We describe a method for the collection and microanalysis of the volatile organic compounds in human breath. A transportable apparatus supplies subjects with purified air and samples their alveolar breath; the volatile organic compounds are captured in an adsorptive trap containing activated carbon and molecular sieve. The sample is thermally desorbed from the trap in an automated microprocessor-controlled device, concentrated by two-stage cryofocusing, and assayed by gas chromatography with ion-trap detection. Compounds are identified by reference to a computer-based library of mass spectra with subtraction of the background components present in the inspired air. We used this device to study 10 normal subjects and determined the relative abundance of the volatile organic compounds in their alveolar breath. The breath-collecting apparatus was convenient to operate and was well tolerated by human volunteers.
我们描述了一种用于收集和微量分析人体呼出气体中挥发性有机化合物的方法。一种可移动装置为受试者提供净化空气并采集其肺泡气；挥发性有机化合物被捕获在一个装有活性炭和分子筛的吸附阱中。样品在一个由微处理器自动控制的装置中从阱中热解吸出来，通过两级低温聚焦进行浓缩，并用带有离子阱检测的气相色谱法进行分析。通过参考基于计算机的质谱库并减去吸入空气中存在的背景成分来鉴定化合物。我们使用该装置研究了10名正常受试者，并确定了他们肺泡气中挥发性有机化合物的相对丰度。该呼气收集装置操作方便，人类志愿者耐受性良好。
