Holzer Hananel, Scharf Eleanor, Chian Ri-Cheng, Demirtas Ezgi, Buckett William, Tan Seang Lin
McGill Reproductive Center, Department of Obstetrics and Gynecology, McGill University, Montreal, Quebec, Canada.
Fertil Steril. 2007 Jul;88(1):62-7. doi: 10.1016/j.fertnstert.2006.11.087. Epub 2007 Mar 6.
To assess the role of immature oocyte collection from unstimulated ovaries as a potential source of oocyte donation.
Prospective cohort study.
A tertiary, university-based, in vitro fertilization center.
PATIENT(S): Twelve oocyte donors with ultrasound-only polycystic ovaries or polycystic ovary syndrome matched with 12 oocyte recipients.
INTERVENTION(S): Immature oocyte collection without any ovarian stimulation. In vitro maturation of the oocytes. Embryo transfer of the embryos.
MAIN OUTCOME MEASURE(S): Immature oocyte collection, maturation, fertilization, and cleavage rates. Implantation, pregnancy, and live birth rates.
RESULT(S): A mean of 12.8 +/- 5.1 Germinal-vesicle oocytes were aspirated per collection. The in vitro maturation rate was 68.3% +/- 18.4% with a mean of 8.7 +/- 3.6 mature oocytes per collection. The mean fertilization rate was 73.3% +/- 19.4%. Two to five embryos (median four) were transferred. Six recipients conceived, giving a 50% clinical pregnancy rate per cycle. The mean implantation rate per embryo was 18.2%. The live birth rate per cycle started was 30%.
CONCLUSION(S): Collecting immature oocytes from unstimulated ovaries for the purpose of oocyte donation is a simple procedure that totally avoids ovarian stimulation. With appropriate selection of women with ultrasound-only polycystic ovaries or women with the polycystic ovary syndrome, the pregnancy rates of the recipients are comparable with those achieved through conventional IVF oocyte donor cycles.
评估从未经刺激的卵巢中采集未成熟卵母细胞作为卵母细胞捐赠潜在来源的作用。
前瞻性队列研究。
一所大学附属的三级体外受精中心。
12名仅通过超声诊断为多囊卵巢或多囊卵巢综合征的卵母细胞捐赠者与12名卵母细胞接受者匹配。
在不进行任何卵巢刺激的情况下采集未成熟卵母细胞。卵母细胞的体外成熟。胚胎移植。
未成熟卵母细胞的采集、成熟、受精和分裂率。着床率、妊娠率和活产率。
每次采集平均吸出12.8±5.1个生发泡期卵母细胞。体外成熟率为68.3%±18.4%，每次采集平均有8.7±3.6个成熟卵母细胞。平均受精率为73.3%±19.4%。移植2至5个胚胎（中位数为4个）。6名接受者受孕，每个周期的临床妊娠率为50%。每个胚胎的平均着床率为18.2%。每个开始周期的活产率为30%。
为了卵母细胞捐赠从未经刺激的卵巢中采集未成熟卵母细胞是一个简单的程序，完全避免了卵巢刺激。通过适当选择仅通过超声诊断为多囊卵巢的女性或多囊卵巢综合征女性，接受者的妊娠率与通过传统体外受精卵母细胞捐赠周期所达到的妊娠率相当。
