使用市售全细胞病毒裂解物、合成肽和重组蛋白酶免疫测定系统检测1型人类免疫缺陷病毒抗体。
Detection of human immunodeficiency virus type 1 antibody by using commercially available whole-cell viral lysate, synthetic peptide, and recombinant protein enzyme immunoassay systems.
作者信息
Johnson J E
机构信息
Serology Reference Laboratory (113A), Veterans Administration Medical Center, Lexington, Kentucky 40511.
出版信息
J Clin Microbiol. 1992 Jan;30(1):216-8. doi: 10.1128/jcm.30.1.216-218.1992.
Abstract
A total of 575 serum specimens received for human immunodeficiency virus type 1 (HIV-1) antibody determination were tested prospectively by enzyme immunoassay with a whole-cell viral lysate (VL) (Genetic Systems Corp.), a synthetic peptide (SP) (United Biomedical, Inc.), and a recombinant protein (RCP) (Syva Co.). Concordance of all three antigens was noted for 559 of 575 (97.2%) specimens tested. Of the specimens tested, 90 (15.7%) were positive and 469 (84.3%) were negative. Retrospective testing by SP and RCP of 86 specimens indeterminate for HIV-1 antibody by VL, Western blot (immunoblot), and immunofluorescence was also performed. The results of both phases of this study indicate that the specificity of the three antigens is RCP greater than SP greater than VL. As noted from the prospective phase, the sensitivities of the antigens appear to be equivalent, indicating that the RCP and SP systems could be used in place of, or to confirm, a VL enzyme immunoassay screening test.
摘要
共有575份用于检测1型人类免疫缺陷病毒（HIV-1）抗体的血清标本，采用全细胞病毒裂解物（VL）（基因系统公司）、合成肽（SP）（联合生物医学公司）和重组蛋白（RCP）（赛瓦公司）通过酶免疫测定法进行前瞻性检测。在检测的575份标本中，有559份（97.2%）的所有三种抗原结果一致。在检测的标本中，90份（15.7%）为阳性，469份（84.3%）为阴性。还对86份通过VL、免疫印迹和免疫荧光检测HIV-1抗体结果不确定的标本进行了SP和RCP回顾性检测。本研究两个阶段的结果表明，三种抗原的特异性为RCP大于SP大于VL。如前瞻性阶段所示，抗原的敏感性似乎相当，这表明RCP和SP系统可用于替代VL酶免疫测定筛查试验或对其进行确认。
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