Lepine D G, Neumann P W, Frenette S L, O'Shaughnessy M V
Bureau of Laboratories and Research, Federal Centre for AIDS, Ottawa, Ontario, Canada.
J Clin Microbiol. 1990 Jun;28(6):1169-71. doi: 10.1128/jcm.28.6.1169-1171.1990.
Many truly human immunodeficiency virus (HIV) antibody-negative serum samples may be unnecessarily subjected to costly and time-consuming Western blots (immunoblots). An investigation was undertaken to evaluate the efficiency of using a recombinant protein-based enzyme immunosorbent assay (EIA; Cambridge BioScience [CBC] Recombigen HIV EIA) as an adjunct to whole viral lysate EIA. A total of 2,212 serum samples which had been screened by viral lysate EIA were tested by CBC EIA in parallel with the Western blot. The sensitivity and specificity of the CBC kit were 99.9 and 99.7%, respectively. Positive and negative predictive values were 99.7 and 99.9%, respectively. The high sensitivity of this kit and its high negative predictive value make it an attractive addition to an HIV testing algorithm by reducing the number of Western blot tests on truly antibody negative serum samples.
许多真正的人类免疫缺陷病毒（HIV）抗体阴性血清样本可能会不必要地接受昂贵且耗时的蛋白质印迹法（免疫印迹法）检测。开展了一项调查，以评估使用基于重组蛋白的酶联免疫吸附测定（EIA；剑桥生物科学公司[CBC]重组HIV EIA）作为全病毒裂解物EIA辅助检测方法的效率。共有2212份经病毒裂解物EIA筛查的血清样本，同时采用CBC EIA和蛋白质印迹法进行检测。CBC试剂盒的灵敏度和特异性分别为99.9%和99.7%。阳性预测值和阴性预测值分别为99.7%和99.9%。该试剂盒的高灵敏度及其高阴性预测值，通过减少对真正抗体阴性血清样本进行蛋白质印迹检测的数量，使其成为HIV检测方案中一个有吸引力的补充方法。
