Burke D S, Brandt B L, Redfield R R, Lee T H, Thorn R M, Beltz G A, Hung C H
Ann Intern Med. 1987 May;106(5):671-6. doi: 10.7326/0003-4819-106-5-671.
To detect human immunodeficiency virus (HIV) antibodies in a simple enzyme-linked immunoassay (CBre3-EIA), we used an Escherichia coli-expressed polypeptide antigen, representing the carboxy-terminal third of the external membrane glycoprotein gene fused with the amino-terminal half of the transmembrane glycoprotein gene. Over a 3-month period, 2707 consecutive serum samples referred for confirmatory testing for human T-lymphotrophic virus type III (HTLV-III) antibodies were evaluated by both Western blot and CBre3-EIA. On a single determination for each sample, the CBre3-EIA was found to have an estimated sensitivity (99.9%) and specificity (99.1%) similar or superior to the more cumbersome Western blot method. This study shows that all HIV-seropositive subjects have antibodies to the virus envelope protein; no other virus antigens are required for construction of highly sensitive immunoassays.
为了在一种简单的酶联免疫测定法（CBre3-EIA）中检测人类免疫缺陷病毒（HIV）抗体，我们使用了一种在大肠杆菌中表达的多肽抗原，该抗原代表外膜糖蛋白基因羧基末端的三分之一与跨膜糖蛋白基因氨基末端的一半融合。在3个月的时间里，对2707份连续送检进行人类嗜T淋巴细胞病毒III型（HTLV-III）抗体确证检测的血清样本，同时采用免疫印迹法和CBre3-EIA进行评估。对每个样本单次测定时，发现CBre3-EIA的估计灵敏度（99.9%）和特异性（99.1%）与更繁琐的免疫印迹法相似或更优。这项研究表明，所有HIV血清阳性受试者都具有针对病毒包膜蛋白的抗体；构建高灵敏度免疫测定法无需其他病毒抗原。
