Department of Obstetrics and Gynecology, Faculty of Medicine, Universitas Airlangga, Surabaya, Indonesia.
Department of Veterinary Science, Faculty of Veterinary Medicine, Universitas Airlangga, Surabaya, Indonesia.
Open Vet J. 2023 Mar;13(3):288-296. doi: 10.5455/OVJ.2023.v13.i3.4. Epub 2023 Mar 7.
Kisspeptin is a neuropeptide that has an important role in the female reproductive cycle which is indicated by its role in regulating the hypothalamic-pituitary-gonadal axis.
To analyze the correlation between serum kisspeptin levels, ovarian kisspeptin expression, and ovarian Bone Morphogenic Protein-15 (BMP15) expression in polycystic ovary syndrome (PCOS) model rats.
The research was accurate experimental research with a post-test design-only control group and was carried out from August to October 2022 at the Faculty of Veterinary Medicine Universitas Airlangga. 32 rats were divided into a control group and a PCOS model group. Blood serum and ovaries were obtained from all groups. In addition, blood serum was examined for kisspeptin levels by ELISA technique, and kisspeptin expression and BMP15 Ovaries were examined immunohistochemically.
Serum kisspeptin levels and ovarian kisspeptin expression of the PCOS model group were not significantly higher than those of the control group ( > 0.05, > 0.05). The ovarian BMP15 expression of the PCOS model group was not significantly lower ( > 0.05) than that of the control group. Ovarian kisspeptin expression and ovarian BMP15 expression did not significantly correlate with serum kisspeptin levels ( > 0.05). In contrast, there was a significant correlation ( < 0.05) between ovarian kisspeptin expression and ovarian BMP15 expression.
Serum kisspeptin levels and ovarian kisspeptin expression of the PCOS model group were not higher than those of the control group, and the ovarian BMP15 expression of the PCOS model group was not lower than that of the control group. There was no correlation between serum kisspeptin levels with ovarian kisspeptin expression and ovarian BMP15 expression. However, a significant correlation was found between ovarian kisspeptin expression and ovarian BMP15 expression.
kisspeptin 是一种神经肽，在女性生殖周期中发挥着重要作用，其作用是调节下丘脑-垂体-性腺轴。
分析多囊卵巢综合征（PCOS）模型大鼠血清 kisspeptin 水平、卵巢 kisspeptin 表达与卵巢骨形态发生蛋白 15（BMP15）表达的相关性。
这是一项准确的实验研究，采用后测试设计仅对照组，于 2022 年 8 月至 10 月在 Airlangga 大学兽医学院进行。32 只大鼠分为对照组和 PCOS 模型组。从所有组中获取血清和卵巢。此外，通过 ELISA 技术检测血清 kisspeptin 水平，免疫组织化学法检测卵巢 kisspeptin 表达和 BMP15。
PCOS 模型组血清 kisspeptin 水平和卵巢 kisspeptin 表达均未显著高于对照组（>0.05，>0.05）。PCOS 模型组卵巢 BMP15 表达与对照组相比无显著降低（>0.05）。卵巢 kisspeptin 表达与卵巢 BMP15 表达与血清 kisspeptin 水平无显著相关性（>0.05）。相反，卵巢 kisspeptin 表达与卵巢 BMP15 表达之间存在显著相关性（<0.05）。
PCOS 模型组血清 kisspeptin 水平和卵巢 kisspeptin 表达均未高于对照组，PCOS 模型组卵巢 BMP15 表达亦未低于对照组。血清 kisspeptin 水平与卵巢 kisspeptin 表达和卵巢 BMP15 表达之间无相关性。然而，卵巢 kisspeptin 表达与卵巢 BMP15 表达之间存在显著相关性。
