Alfieri Alessio, Maione Francesco, Bisio Angela, Romussi Giovanni, Mascolo Nicola, Cicala Carla
Dipartimento di Farmacologia Sperimentale, University of Naples Federico II, via D. Montesano 49, 80131 Napoli, Italy.
Phytother Res. 2007 Jul;21(7):690-2. doi: 10.1002/ptr.2128.
The effect of a diterpenoid isolated from Salvia cinnabarina, 3,4-seicosopimar-4(18),7,15-triene-3-oic acid (SCB), on arterial blood pressure was evaluated in anaesthetized rats. Male Wistar rats, anaesthetized with urethane (sol. 10% p/v; 10 mL/kg), underwent surgery for continuous monitoring of arterial blood pressure. After preliminary experiments to evaluate the dose response (3, 10 and 30 mg/kg i.v.) of SCB, a dose of 3 mg/kg was chosen for all successive experiments. On different groups of rats treated with the ganglion-blocking agent chlorisondamine (2.5 mg/kg i.p.) the effect of SCB (3 mg/kg i.v.) was evaluated before and following an infusion of the nitric oxide synthase inhibitor L-NAME (0.3 mg/kg/min i.v.). Intravenous administration of SCB at doses of 3, 10 and 30 mg/kg led to a fall in mean arterial blood pressure (MABP) of 14.75 +/- 1.44 mmHg, 36.60 +/- 31.40 mmHg and 31.40 +/- 6.28 mmHg, respectively (n = 4-5), that was not modified by treatment of the rat with chlorisondamine nor with L-NAME. The results demonstrate a hypotensive effect of SCB - due to a peripheral mechanism but independent of endothelial nitric oxide release.
评估了从朱唇鼠尾草中分离得到的二萜类化合物3,4-二十二碳-4(18),7,15-三烯-3-酸（SCB）对麻醉大鼠动脉血压的影响。雄性Wistar大鼠用乌拉坦（10% p/v溶液；10 mL/kg）麻醉后，进行手术以连续监测动脉血压。在初步实验评估了SCB的剂量反应（静脉注射3、10和30 mg/kg）后，所有后续实验均选择3 mg/kg的剂量。在不同组用神经节阻断剂氯异吲哚铵（腹腔注射2.5 mg/kg）处理的大鼠中，在静脉注射一氧化氮合酶抑制剂L-NAME（静脉注射0.3 mg/kg/min）之前和之后评估了SCB（静脉注射3 mg/kg）的作用。静脉注射3、10和30 mg/kg剂量的SCB分别导致平均动脉血压（MABP）下降14.75±1.44 mmHg、36.60±31.40 mmHg和31.40±6.28 mmHg（n = 4 - 5），用氯异吲哚铵或L-NAME处理大鼠均未改变这一结果。结果表明SCB具有降压作用——归因于外周机制，但与内皮一氧化氮释放无关。
