Keskin Derin B, Allan David S J, Rybalov Basya, Andzelm Milena M, Stern Joel N H, Kopcow Hernan D, Koopman Louise A, Strominger Jack L
Department of Molecular and Cellular Biology, Harvard University, Cambridge, MA 02138, USA.
Proc Natl Acad Sci U S A. 2007 Feb 27;104(9):3378-83. doi: 10.1073/pnas.0611098104. Epub 2007 Feb 20.
During pregnancy the uterine decidua is populated by large numbers of natural killer (NK) cells with a phenotype CD56(superbright)CD16(-)CD9(+)KIR(+) distinct from both subsets of peripheral blood NK cells. Culture of highly purified CD16(+)CD9(-) peripheral blood NK cells in medium containing TGFbeta1 resulted in a transition to CD16(-)CD9(+) NK cells resembling decidual NK cells. Decidual stromal cells, when isolated and cultured in vitro, were found to produce TGFbeta1. Incubation of peripheral blood NK cells with conditioned medium from decidual stromal cells mirrored the effects of TGFbeta1. Similar changes may occur upon NK cell entry into the decidua or other tissues expressing substantial TGFbeta. In addition, Lin(-)CD34(+)CD45(+) hematopoietic stem/progenitor cells could be isolated from decidual tissue. These progenitors also produced NK cells when cultured in conditioned medium from decidual stromal cells supplemented with IL-15 and stem cell factor.
在妊娠期间，子宫蜕膜中有大量自然杀伤（NK）细胞，其表型为CD56（超亮）CD16（-）CD9（+）KIR（+），与外周血NK细胞的两个亚群均不同。在含有转化生长因子β1（TGFβ1）的培养基中培养高度纯化的CD16（+）CD9（-）外周血NK细胞，会导致其转变为类似于蜕膜NK细胞的CD16（-）CD9（+）NK细胞。体外分离培养的蜕膜基质细胞被发现可产生TGFβ1。用蜕膜基质细胞的条件培养基孵育外周血NK细胞，可反映出TGFβ1的作用。当NK细胞进入蜕膜或其他表达大量TGFβ的组织时，可能会发生类似的变化。此外，可从蜕膜组织中分离出Lin（-）CD34（+）CD45（+）造血干细胞/祖细胞。当在添加了白细胞介素15（IL-15）和干细胞因子的蜕膜基质细胞条件培养基中培养时，这些祖细胞也会产生NK细胞。
