Kumari Jaya, Sahoo P K, Giri S S
Central Institute of Freshwater Aquaculture, Kausalyaganga, Bhubaneswar 751 002, India.
Indian J Exp Biol. 2007 Mar;45(3):291-8.
A series of experiments were performed to determine the impact of polyherbal immunomodulatory formulation 'ImmuPlus' (AquaImmu) on growth, immunity and disease resistance of rohu (Labeo rohita), one of the Indian major carp at different stages of growth. Rohu larvae were fed on plankton, ImmuPlus-mixed compound feed, and plankton plus ImmuPlus-mixed compound feed (ImmuPlus added at three dose levels of 0.25, 0.50, and 0.75 g/kg feed) from 4th day of hatching to 14th day. ImmuPlus-mixed diets enhanced growth of larvae, survival and disease resistance against Aeromonas hydrophila challenge, compared to only plankton-fed group. In two other experiments, advanced rohu larvae and fingerlings were fed with ImmuPlus-mixed compound feed (at 0, 0.5, 1.0 and 2.0 g/kg) for 15, 30 and 45 days. At the end of 45 days for advanced larvae and 30 days for fingerlings, the fish fed with ImmuPlus at 1.0 g/kg level showed significantly higher growth and disease resistance against A. hydrophila challenge. In a separate experiment, juveniles of rohu were fed with 1 g/kg of ImmuPlus incorporated feed for 15 and 30 days. At the end of the trial, the ImmuPlus fed fish showed enhanced non-specific immunity (as measured through nitroblue tetrazolium reduction assay, serum lysozyme activity, serum haemolysin titre and resistance against A. hydrophila challenge in non-vaccinated fish as well as specific immunity levels (as measured through bacterial agglutination titre against A. hydrophila in vaccinated fish). Incorporation of ImmuPlus at 1 g/kg level in the diet of rohu may be beneficial for enhancing disease resistance.
进行了一系列实验，以确定多草药免疫调节制剂“ImmuPlus”（AquaImmu）对印度主要鲤鱼品种之一的露斯塔野鲮（Labeo rohita）在不同生长阶段的生长、免疫力和抗病性的影响。从孵化后第4天到第14天，给露斯塔野鲮幼鱼投喂浮游生物、添加ImmuPlus的复合饲料，以及浮游生物加添加ImmuPlus的复合饲料（ImmuPlus添加量为0.25、0.50和0.75 g/kg饲料三个剂量水平）。与仅投喂浮游生物的组相比，添加ImmuPlus的饲料提高了幼鱼的生长、存活率以及对嗜水气单胞菌攻击的抗病性。在另外两个实验中，给大龄露斯塔野鲮幼鱼和鱼种投喂添加ImmuPlus的复合饲料（添加量为0、0.5、1.0和2.0 g/kg），持续15、30和45天。在大龄幼鱼实验45天结束时以及鱼种实验30天结束时，投喂1.0 g/kg水平ImmuPlus的鱼生长显著加快，对嗜水气单胞菌攻击的抗病性也显著增强。在一项单独实验中，给露斯塔野鲮幼鱼投喂添加1 g/kg ImmuPlus的饲料，持续15和30天。实验结束时，投喂ImmuPlus的鱼显示出非特异性免疫力增强（通过硝基蓝四氮唑还原试验、血清溶菌酶活性、血清溶血素效价测定，以及未接种疫苗的鱼对嗜水气单胞菌攻击的抗性），以及特异性免疫水平提高（通过接种疫苗的鱼对嗜水气单胞菌的细菌凝集效价测定）。在露斯塔野鲮饲料中添加1 g/kg水平的ImmuPlus可能有利于增强抗病性。
