Hokugo Akishige, Sawada Yasunori, Hokugo Rie, Iwamura Hiromitsu, Kobuchi Masakiyo, Kambara Toshiyuki, Morita Shosuke, Tabata Yasuhiko
Department of Biomaterials, Field of Tissue Engineering, Institute for Frontier Medical Sciences, Kyoto University, Kawara-cho Shogoin, Sakyo-ku, Kyoto, Japan.
Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2007 Jul;104(1):44-8. doi: 10.1016/j.tripleo.2006.11.032. Epub 2007 Mar 26.
Platelet-rich plasma (PRP) has been clinically employed to promote bone regeneration. However, few studies have investigated the enhancement of biological function of platelet growth factors after integration of PRP into biomaterials. In this study, the feasibility of gelatin hydrogels for controlled release of platelet growth factors and the consequent enhancement of PRP-induced bone regeneration were evaluated in rabbit calvarial defect.
Gelatin hydrogels incorporating PRP, PRP-activated thrombin, or an empty gelatin hydrogel were applied to the defect, or the defect was left untreated. Bone regeneration was evaluated by microfocus computed tomography, peripheral quantitative computed tomography, and histological examinations.
Successful bone regeneration was observed at the bone defect applied with the gelatin hydrogel incorporating PRP, which is in marked contrast to other groups.
The gelatin hydrogel is a promising material capable of controlled release of platelet growth factors to enhance bone regeneration.
富血小板血浆（PRP）已在临床上用于促进骨再生。然而，很少有研究探讨PRP与生物材料结合后血小板生长因子生物学功能的增强情况。在本研究中，在兔颅骨缺损模型中评估了明胶水凝胶用于控制释放血小板生长因子以及随之增强PRP诱导的骨再生的可行性。
将含有PRP、PRP激活的凝血酶的明胶水凝胶或空白明胶水凝胶应用于缺损处，或不进行处理。通过微焦点计算机断层扫描、外周定量计算机断层扫描和组织学检查评估骨再生情况。
在应用含有PRP的明胶水凝胶的骨缺损处观察到成功的骨再生，这与其他组形成显著对比。
明胶水凝胶是一种有前景的材料，能够控制释放血小板生长因子以增强骨再生。
