de Ruiter Maaike T, de Valk Hanneke A, Meis Jacques F G M, Klaassen Corné H W
Department of Medical Microbiology and Infectious Diseases, Canisius Wilhelmina Hospital, Weg door Jonkerbos 100, 6532 SZ Nijmegen, The Netherlands.
J Microbiol Methods. 2007 Sep;70(3):528-34. doi: 10.1016/j.mimet.2007.06.009. Epub 2007 Jun 27.
Retrotransposon(-like) sequences in Aspergillus fumigatus have been used as typing targets through restriction fragment length polymorphism (RFLP)/Southern blotting approaches. Differences in fingerprints between unrelated isolates are the result of variations in copy-number and differences in the regions flanking the retrotransposon elements. Here, we present retrotransposon insertion-site context (RISC) typing as a novel and convenient PCR-based typing alternative to the RFLP approach. RISC typing aims at amplifying the sequences flanking the retrotransposon-like sequences in A. fumigatus and allows large numbers of isolates to be analyzed in a timely fashion with excellent discriminatory power.
烟曲霉中的反转录转座子（类）序列已通过限制性片段长度多态性（RFLP）/Southern印迹法用作分型靶点。不相关菌株之间指纹图谱的差异是反转录转座子元件侧翼区域拷贝数变化和差异的结果。在此，我们提出反转录转座子插入位点背景（RISC）分型，作为一种新颖且便捷的基于PCR的分型方法，可替代RFLP方法。RISC分型旨在扩增烟曲霉中反转录转座子样序列侧翼的序列，并能够及时对大量菌株进行分析，具有出色的鉴别能力。
