Cachay Edward R, Frost Simon D W, Richman Douglas D, Smith Davey M, Little Susan J
University of California, San Diego, La Jolla, CA 92103, USA.
J Infect Dis. 2007 May 1;195(9):1270-7. doi: 10.1086/513568. Epub 2007 Mar 15.
We sought to compare baseline and longitudinal plasma HIV-1 loads between herpes simplex virus type 2 (HSV-2)-seropositive and -seronegative individuals who are enrolled in a primary HIV-1 infection cohort in San Diego, California.
The study was a retrospective cohort analysis.
We categorized antiretroviral-naive subjects on the basis of HSV-2 serostatus at baseline using an HSV-2 enzyme immunoassay. Low positive results (1.1-3.5) were confirmed by Western blotting. We compared baseline HIV-1 loads of the 2 groups using a linear model. To detect differences in HIV-1 dynamics, we analyzed longitudinal viral loads using a flexible semiparametric model, controlling for the time to antiretroviral therapy and stratifying by HIV-1 infection stage at entry.
We studied 294 adult men. Ninety percent reported sex with men as their main HIV-1 risk factor. The seroprevalence of HSV-2 was 41.5%. The HSV-2-seropositive and -seronegative groups had similar baseline HIV-1 loads during acute infection (5.52 vs. 5.72 log(10) copies/mL; P=.39) and early infection (4.57 vs. 4.67 log(10) copies/mL; P=.5). Longitudinally, the difference in HIV-1 loads between HSV-2-seropositive and -seronegative men remained close to 0 during the first year of infection.
HSV-2 serostatus has minimal influence on the dynamics of HIV-1 during acute and early HIV-1 infection.
我们试图比较加利福尼亚州圣地亚哥一个原发性HIV-1感染队列中，2型单纯疱疹病毒（HSV-2）血清学阳性和血清学阴性个体的基线和纵向血浆HIV-1载量。
该研究为回顾性队列分析。
我们使用HSV-2酶免疫测定法，根据基线时的HSV-2血清状态，将未接受过抗逆转录病毒治疗的受试者进行分类。低阳性结果（1.1 - 3.5）通过蛋白质印迹法确认。我们使用线性模型比较两组的基线HIV-1载量。为了检测HIV-1动态变化的差异，我们使用灵活的半参数模型分析纵向病毒载量，控制抗逆转录病毒治疗时间，并按入组时的HIV-1感染阶段进行分层。
我们研究了294名成年男性。90%报告称与男性发生性行为是其主要的HIV-1危险因素。HSV-2血清阳性率为41.5%。HSV-2血清阳性和血清阴性组在急性感染期间（5.52对5.72 log₁₀拷贝/毫升；P = 0.39）和早期感染期间（4.57对4.67 log₁₀拷贝/毫升；P = 0.5）的基线HIV-1载量相似。纵向来看，在感染的第一年，HSV-2血清阳性和血清阴性男性之间的HIV-1载量差异仍接近0。
在急性和早期HIV-1感染期间，HSV-2血清状态对HIV-1动态变化的影响极小。
