Brattain M G, Kimball P M, Pretlow T G, Marks M E
Biochem J. 1977 May 1;163(2):247-51. doi: 10.1042/bj1630247.
The specific interaction between human N-acetylhexosaminidase and concanavalin A was evaluated with respect to temperature, time, pH and concentration of specific ligand in incubation mixtures containing the enzyme and insolubilized lectin. Elution of the enzyme from insolubilized concanavalin A is dependent on both temperature and concentration of alpha-methyl mannoside. Conditions for a high yield of the enzyme from chromatography on insolubilized concanavalin A are described.
在含有该酶和固定化凝集素的孵育混合物中,针对温度、时间、pH值和特定配体的浓度,评估了人N - 乙酰己糖胺酶与伴刀豆球蛋白A之间的特异性相互作用。从固定化伴刀豆球蛋白A上洗脱该酶取决于α - 甲基甘露糖苷的温度和浓度。描述了在固定化伴刀豆球蛋白A上进行色谱分离以高产率获得该酶的条件。