Li Qian, Liu Qing-Hui, Huang Jie
Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao, 266071 China ; Shanghai Ocean University, Shanghai, China.
Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao, 266071 China.
Indian J Virol. 2013 Jun;24(1):54-8. doi: 10.1007/s13337-012-0111-2. Epub 2012 Dec 16.
Interactions between virus structural proteins are suggested to be crucial for virus assembly. Many steps in the process of white spot syndrome virus (WSSV) assembly and maturation remain unclear. In this paper, we discovered a new interaction of WSSV VP292. Temporal-transcription analysis showed that VP292 is expressed in the late stage of WSSV infection. Western blot and matrix-assisted laser desorption ionization MS assays showed that VP292 interacts with VP26, a major envelope protein. Far-western blot provided further evidence for interaction between VP292 and VP26. These results collectively demonstrated that VP292 anchors to the envelope through interaction with VP26.
病毒结构蛋白之间的相互作用被认为对病毒组装至关重要。白斑综合征病毒（WSSV）组装和成熟过程中的许多步骤仍不清楚。在本文中，我们发现了WSSV VP292的一种新相互作用。时序转录分析表明，VP292在WSSV感染后期表达。蛋白质免疫印迹和基质辅助激光解吸电离质谱分析表明，VP292与一种主要包膜蛋白VP26相互作用。蛋白质印迹法进一步证明了VP292与VP26之间的相互作用。这些结果共同表明，VP292通过与VP26相互作用锚定在包膜上。
