在Tc1切除后，通过转基因指导的DNA双链断裂修复对秀丽隐杆线虫基因组进行靶向改造。

Plasterk R H, Groenen J T

Netherlands Cancer Institute, Division of Molecular Biology, Amsterdam.

EMBO J. 1992 Jan;11(1):287-90. doi: 10.1002/j.1460-2075.1992.tb05051.x.

Excision of a Tc1 transposon of Caenorhabditis elegans is thought to leave a DNA double strand break. We report here that sequence polymorphisms in a transgenic DNA template are copied into the corresponding chromosomal gene upon excision of Tc1 from the chromosome. This shows that the double strand DNA break resulting from Tc1 excision is repaired with the extrachromosomal DNA as template and that sequences flanking the break can be replaced by sequences from the transgene. Transgene instructed break repair provides a method for the targeted introduction of precise alterations into the Caenorhabditis elegans genome.

秀丽隐杆线虫Tc1转座子的切除被认为会留下DNA双链断裂。我们在此报告，当Tc1从染色体上切除时，转基因DNA模板中的序列多态性会被复制到相应的染色体基因中。这表明，以染色体外DNA为模板修复了由Tc1切除导致的双链DNA断裂，并且断裂侧翼的序列可以被来自转基因的序列所取代。转基因指导的断裂修复为将精确改变靶向引入秀丽隐杆线虫基因组提供了一种方法。