利用红外激光光镊对人类精子进行三维可控微操作：对精子速度的影响

Controlled micromanipulation of human sperm in three dimensions with an infrared laser optical trap: effect on sperm velocity.

作者信息

Colon J M, Sarosi P, McGovern P G, Askin A, Dziedzic J M, Skurnick J, Weiss G, Bonder E M

机构信息

Department of Obstetrics and Gynecology, University of Medicine and Dentistry of New Jersey-New Jersey Medical School, Rutgers State University of New Jersey, Newark.

出版信息

Fertil Steril. 1992 Mar;57(3):695-8. doi: 10.1016/s0015-0282(16)54926-7.

DOI:10.1016/s0015-0282(16)54926-7

PMID:1740221

Abstract

Individual human sperm can be micromanipulated in three dimensions using a 1.06 microns Nd:YAG laser trap. Single sperm swimming with velocities in the range of 65 to 85 microns/sec can be trapped with 40 mW of power through 120 seconds without a deleterious effect on velocity. Even though it will be necessary to further evaluate the effects of laser light on specific functions of sperm, our data suggest that decreasing the time of manipulation to a minimum will increase the safety of the micromanipulation procedures. Laser traps may play a role in assisted reproductive technology by facilitating the selective transport of individual sperm.

摘要

利用1.06微米的钕:钇铝石榴石激光阱可以在三维空间对单个人类精子进行显微操作。速度在65至85微米/秒范围内游动的单个精子，可通过40毫瓦的功率捕获120秒，而不会对其速度产生有害影响。尽管有必要进一步评估激光对精子特定功能的影响，但我们的数据表明，将操作时间减至最短将提高显微操作程序的安全性。激光阱可能通过促进单个精子的选择性运输在辅助生殖技术中发挥作用。