Guttman-Yassky Emma, Abada Rinat, Kra-Oz Zipi, Sattinger Judith, Perelman Alex, Bergman Reuven, Sarid Ronit
The Mina and Everard Goodman Faculty of Life Sciences, Bar-Ilan University, Ramat-Gan 52900, Israel.
Diagn Microbiol Infect Dis. 2007 Apr;57(4):387-92. doi: 10.1016/j.diagmicrobio.2006.09.012.
Human herpesvirus 8 (HHV-8), also known as Kaposi sarcoma (KS)-associated herpesvirus, is causally implicated in all forms of KS, including the classic form. Our objective was to investigate the relationship between HHV-8 load in peripheral blood mononuclear cells (PBMCs) and the stage of the disease in classic KS (CKS) patients. HHV-8 loads were measured in 41 PBMC samples from CKS patients with different Krigel-based classification stages using a quantitative real-time polymerase chain reaction assay. Low HHV-8 DNA loads reaching a maximum of 75.5 copies/10(5) cells were detected in 73.2% of the patients. HHV-8 loads in patients with stages I and II were similarly distributed. An increased detection rate of HHV-8 DNA, although not statistically significant, was evident in patients diagnosed with CKS stages III and IV. We conclude that the measurements of HHV-8 load in PBMCs provide a limited correlation with the clinical stage of KS.
人类疱疹病毒8型（HHV - 8），也称为卡波西肉瘤（KS）相关疱疹病毒，与包括经典型在内的所有形式的KS都有因果关系。我们的目的是研究外周血单个核细胞（PBMC）中HHV - 8载量与经典KS（CKS）患者疾病分期之间的关系。使用定量实时聚合酶链反应测定法，对41例来自不同基于Krigel分类分期的CKS患者的PBMC样本中的HHV - 8载量进行了测量。在73.2%的患者中检测到低HHV - 8 DNA载量，最高达到75.5拷贝/10(5)个细胞。I期和II期患者的HHV - 8载量分布相似。在诊断为CKS III期和IV期的患者中，HHV - 8 DNA的检出率有所增加，尽管无统计学意义。我们得出结论，PBMC中HHV - 8载量的测量与KS的临床分期相关性有限。
